阴虚火旺型口腔扁平苔藓miRNAs表达特征研究  

STUDY ON FEATURE OF MIRNAS EXPRESSION IN ORAL LICHEN PLANUS OF HYPERACTIVITY FIRE DUE TO YIN DEFICIENCY TYPE

作　　者：颜家渝[1,2] 丁显平[1] 黄映红[2] 曾洁萍[2] 

机构地区：[1]四川大学生命科学学院遗传医学研究所生物资源与生态环境教育部重点实验室,成都610041 [2]成都中医药大学

出　　处：《现代预防医学》2010年第21期4121-4122,共2页Modern Preventive Medicine

基　　金：四川省科技厅科技支撑计划项目(2008SZ0245);成都中医药大学校基金重点项目(ZRZD200807);成都中医药大学附属医院科技发展基金项目(2009-D-YY-09)

摘　　要：[目的]探索口腔扁平苔藓的中医证候研究方法,探寻阴虚火旺型口腔扁平苔藓的miRNAs表达特征。[方法]选取3例阴虚火旺型口腔扁平苔藓患者及3例正常人血液,分离提取miRNAs,荧光标记后与miRNAs基因芯片杂交,SAM软件筛选阴虚火旺型口腔扁平苔藓患者和正常人有表达差异的miRNA。[结果]获得5个口腔扁平苔藓特异表达miRNAs,其中3个下调,2个上调。[结论]基因芯片技术可用于口腔扁平苔藓中医证候研究,hsa-miR-18a上调和hsa-miR-99b下调可能是阴虚火旺型口腔扁平苔藓的标志性miRNAs。[Objective] To observe the method of researching TCM syndrome of OLP,and to identify the specific miRNAs associated with OLP of hyperactivity fire due to yin deficiency type.[Methods] The miRNAs were extracted and isolated from six samples of blood,including 3 cases with OLP of hyperactivity fire due to yin deficiency type and 3 cases of the control group.miRNAs microarrays were used for screening the expression differences of miRNAs.Data were analyzed using SAM software.[Results] A total of 5 differentially expressed miRNAs were found to be associated with OLP,3 miRNAs were downregulated,2 miRNAs were up-regulated.[Conclusion] Gene chip could be used for the research on TCM syndrome of OLP,miR-18a and miR-99b may be act as the key regulators of gene expression in OLP and correlated with pathogenesis of OLP.

关 键 词：口腔扁平苔藓 miRNAs基因芯片 阴虚火旺 中医证候 

分 类 号：R781.5[医药卫生—口腔医学]