参麦注射液细菌内毒素检测方法的研究  

Bacterial endotoxins test for Shenmai injection by dynamic turbidimetric assay

作　　者：陈华龙 王莉蓓 姜丽君 

机构地区：[1]广东韶关市药品检验所,韶关512028

出　　处：《海峡药学》2010年第10期54-55,共2页Strait Pharmaceutical Journal

摘　　要：目的应用动态浊度法定量检测参麦注射液中的细菌内毒素。方法采用《中国药典》2005年版二部附录细菌内毒素定量检测法。结果将参麦注射液进行1∶20稀释,可排除干扰,回收率在50%-200%,无干扰作用。结论应用动态浊度法测定参麦注射液中的细菌内毒素是可行的,此方法既可避免家兔热原检查法操作复杂的缺点,同时又避免了限量法检测内毒素的干扰。OBJECTIVE To establish a determination of endotoxin in Shenmai injection by dynamic turbidimetric assay.METHODS Inhibition or enhancement test for bacterial endotoxins test were used according to Chp 2005.RESULTS 1∶20 solution of Shenmai Injection showed no interference on test and the recovery was 50%～200%.CONCLUSION Bacterial endotoxin test for Shenmai injection by turbidimetric assay has been established and could be used as an altermative for the rabbit pyrogen test.

关 键 词：动态浊度法 参麦注射液 细菌内毒素 干扰实验 

分 类 号：R927.2[医药卫生—药学]