红细胞血型糖蛋白A位点正常变异及肿瘤放疗的影响  

Variant Frequencies at Glycophorin A Locus in Normal Human Erythrocytes and Effects of Radiotherapy

作　　者：桂红星[1] 程文英[1] 罗伟华 朱涵能[1] 王香娥[1] 丁雅琴 

机构地区：[1]上海医科大学放射医学研究所第三研究室

出　　处：《上海医科大学学报》1999年第2期91-94,共4页Journal of Fudan University(Medical Science)

基　　金：国家教委博土点基金

摘　　要：目的了解血型糖蛋白Ａ（ＧＰＡ）背景突变水平，观察照射近期的变化规律。方法在正常人和肿瘤放疗病人中进行ＧＰＡ突变分析，结合单克隆抗体标记和流式细胞技术，检测ＭＮ杂合子个体中罕见的变异细胞ＮＯ和ＮＮ，确定变异率。结果对２８个正常成人的初步测定，ＮＯ／ＮＮ变异率（Ｖａｒｉａｎｔｆｒｅｑｕｅｎｃｙ，Ｖｆ）（８．３±４．１３）／（５．５０±３．７７）×１０－６；ＮＮ变异率与年龄相关性接近显著意义（Ｐ＝０．０５７），男女之间、吸烟与不吸烟者均无显著差异。随访肿瘤病人，６例治疗前ＧＰＡ变异率ＮＯ（１２．９±６．６０）×１０－６，ＮＮ（７．７±５．０１）×１０－６，与同年龄者无显著差异。连续盆腔照射１月Ｖｆ已有升高，照射２个月的病人ＮＮＶｆ（１７．５±８．９５）×１０－６，显著高于未照病人（Ｐ＝０．０４６８）。结论正常人ＧＰＡ变异率基本与文献相符，年龄等因素可影响背景水平；病人照射后Ｖｆ变化不一，表明个体对辐射敏感性不同，照射近期主要是ＮＮ的变化，是较晚造血周期细胞突变的表现。PurposeMethods Combination of immunolabeling and flow cytometry(FCM), 28 normal adults and 12 patients (female) were examined,including the follow up of patients before and after therapy. Results GPA NO/NN variant frequencies (Vfs) of normal subjects were (8.3± 4.13 )/(5.50±3.77)×10 -6 .In this group, there was only marginal significant relationship between NN Vf and age ( P= 0.057), and no significant difference between sexes,smokers and nonsmokers.GPA NN/NN Vf of 6 patients before therapy was (12.97 ±6.60)/(7.7±5.01)×10 -6 ,not significantly higher than that of the controls. Variant frequencies have been high after one month of continuous irradiation, and NN Vf of patients who received two months of therapy were significantly higher than the level of six patients before exposure [(17.5±8.95×10 -6 , P= 0.0468] .Changes of GPA Vfs differ among the patients during and after therapy. Conclusions Background levels of GPA mutation are in close agreement with the results of previous documents.Some factors like age may affect GPA Vf.Individuals'susceptibility to radiation is different, and NN is the main mechanism of mutation shortly after irradiation, located at the late periods of erythropoiesis.

关 键 词：红细胞 单合子NO GPA 纯合子NN 肿瘤 放射疗法 

分 类 号：R730.55[医药卫生—肿瘤]