基质金属蛋白酶2和9在子宫内膜样腺癌组织中的表达及临床意义  

Expressions of matrix metalloproteinase-2 and matrix metalloproteinase-9 in endometrioid adenocarcinoma tissues and clinical significances

作　　者：朱丹[1] 盛敏佳[1] 付久园[1] 苏海兰[2] 

机构地区：[1]吉林大学中日联谊医院妇产科,吉林长春130033 [2]江苏省吴江市第一人民医院妇产科,江苏吴江215200

出　　处：《吉林大学学报（医学版）》2010年第5期965-968,F0003,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅科研基金资助课题(20060415-3)

摘　　要：目的:研究基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)在子宫内膜样腺癌组织中的表达及临床意义,探讨其与子宫内膜样腺癌发展及生物学行为的相关性。方法:收集本院102例子宫切除标本,其中60例子宫内膜腺癌按临床分期分为Ⅰ期(26例)、Ⅱ期(19例)和Ⅲ期(15例);按组织学分级分为1级(29例)、2级(19例)和3级(12例);按肌层浸润分为无肌层浸润(8例)、肌层浸润<1/2(11例)和肌层浸润>1/2(41例);按有无淋巴转移分为无淋巴结转移组(54例)及有淋巴结转移组(6例),采用链霉素抗生物素蛋白-过氧化酶(SP)免疫组织化学方法检测组织MMP-2和MMP-9的表达情况。结果:MMP-2和MMP-9在子宫内膜癌组织中的阳性表达率分别为76.7%和80.0%,与正常子宫内膜组织(13.60%)及内膜单纯增生组织(20.00%)比较,差异有显著性(P<0.05);临床Ⅲ期及组织学3级子宫内膜癌组织MMP-2和MMP-9的强阳性表达率高于临床Ⅰ期及1级子宫内膜癌(P<0.05);有肌层浸润及淋巴转移的子宫内膜癌组织中MMP-2和MMP-9的强阳性表达率高于无肌层浸润及淋巴转移的子宫内膜癌(P<0.05)。结论:MMP-2和MMP-9在子宫内膜样腺癌中呈高表达;MMP-2和MMP-9的强阳性表达率与子宫内膜样腺癌临床分期、组织学分级及有无肌层浸润、淋巴转移有关联。Objective To investigate the expressions of matrix metalloproteinase-2 （MMP-2）and matrix metalloproteinase-9 （MMP-9）in endometrial adenocarcinoma tissues and clinical significances and explore the correlation between MMP-2,MMP-9and development and biological behavior of endometrial adenocarcinoma. Methods 102 cases of uterine samples with complete data were selected.60 cases of the endometrial adenocarcinoma were divided into three groups by clinical stage：stageⅠ （26cases）,stageⅡ （19cases）,stage Ⅲ （15cases）;three groups by histological level：level 1 （29cases）,level 2 （19cases）,level 3 （12cases）; three groups by myometrial invasion：non-myometrial invasion （8cases）,myometrial invasion〈1/2（11cases）,myometrial invasion〉1/2 （41cases）;and two groups by lymph node metastasis：non-lymph node metastasis （54cases）and lymph node metastasis （6cases）.S-P immunohistochemistry method was used to detect the expressions of MMP-2and MMP-9.Results The positive rates of MMP-2 and MMP-9in endometrial adenocarcinoma tissues （76.7% and 80.0%）were significantly higher than those in normal uterine tissues （13.60%）and hyperplasia endometrial tissues（20.0%）（P〈0.05）.The strong positive rates of MMP-2and MMP-9in endometrial adenocarcinoma tissues at stageⅢand histological level 3were higher than those at stageⅠ and histological level 1（P〈0.05）.The strong positive rates of MMP-2and MMP-9in endometrial adenocarcinoma tissues with myometrial invasion and lymph node metastasis were obviously higher than those with non-myometrial invasion and non-lymph node metastasis（P〈0.05）.There are significant differences between them according to the statistics analysis（P〈0.05）.Conclusion The positive expression rates of MMP-2and MMP-9in endometrioid adenocarcinoma tissues are high.The strong positive expression rates of MMP-2and MMP-9are related to tumor differentiation grade,clinical stage,myometrial invasion and lymph node mestastasis.

关 键 词：子宫内膜样腺癌 基质金属蛋白酶2 基质金属蛋白酶9 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]