内生青霉菌纤维素酶在银杏总黄酮提取中的应用研究  

Application of a Cellulose-producing Endophytic Penicillium sp. G-6 on Extraction of Total Flavonoids from Ginkgo biloba

作　　者：赵一璐[1] 许云峰[1] 周建芹 陈韶华[1] 王剑文[1] 

机构地区：[1]苏州大学药学院,苏州215123

出　　处：《中国实验方剂学杂志》2010年第16期13-17,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省"六大人才高峰"第五批高层次人才项目(B2008048)

摘　　要：目的:利用筛选的高产纤维素酶内生青霉菌的发酵液辅助提取银杏黄酮。方法:采用单因素分析和正交试验,以银杏总黄酮提取率为指标,对酶辅助提取中影响总黄酮提取效果的主要因素(如酶解时间,温度,pH,酶料比等)进行研究。结果:通过单因素试验和正交试验,得出纤维素酶发酵液酶解银杏叶的最佳条件为:发酵液以酶料比15∶1加入银杏干粉中,在pH5.0、温度50℃下,酶解处理3 h,该条件下的银杏黄酮提取率达到了1.33%,比常规醇提法提高了18.75%,与纯酶辅助提取率相近。结论:内生青霉菌发酵液辅助法是提取银杏总黄酮的有效方法。Objective： To study the application of the crude cellulase in the culture of endophytic Penicillium sp.G-6 to total flavonoids extraction from leaves of Ginkgo biloba.Method：The process was studied by single factor analysis and orthogonal design L9（3^4） with the extraction rate of total flavonoids as comprehensive index,and the effect of the ratio of dry G.biloba leaves to crude enzymatic extracts,temperature,time,and pH value on extraction rate were also studied.Result：The extraction rate of total flavonoids was 1.33%,increased by 18.75% than that in regular extraction process,under the condition that dry G.biloba leaves were incubated with crude enzymatic extracts with 15∶ 1（pH 5.0） at 50 ℃ for 3 h.Conclusion：The results presented a practical method of cellulase-assistant extraction process on total flavonoids in leaves of Ginkgo biloba.

关 键 词：纤维素酶 内生青霉菌 银杏 黄酮 提取 

分 类 号：R283.6[医药卫生—中药学]