双根清脑颗粒对血管性痴呆模型大鼠神经干细胞增殖迁移的影响  

Effect of Shuanggen Qingnao Granules on Proliferation and Migration of Neural Stem Cells in Rat with Vascular Dementia

作　　者：吴颢昕[1] 李辉[1] 岳晓杰[1] 郑鸿燕[1] 

机构地区：[1]南京中医药大学脑病基础研究实验室,南京210029

出　　处：《中国实验方剂学杂志》2010年第15期110-113,116,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省自然科学基金项目(BK2007243)

摘　　要：目的:观察双根清脑颗粒对血管性痴呆(VD)模型大鼠神经干细胞(NSCs)增殖、迁移的影响,探讨双根清脑颗粒治疗VD的机理。方法:SPF级SD大鼠42只,随机分为双根清脑颗粒高、中、低剂量组,脑复康组、模型组、假手术组、正常组等7组,每组大鼠6只。采用注入同种血栓栓子方法造成VD模型,中药高、中、低剂量组分别以双根清脑颗粒36,12,4 g.kg-1 ig,脑复康组以0.108 g.kg-1 ig,给药容积均为1.5 mL.kg-1,模型组、假手术组和正常组以等量生理盐水ig。免疫组化法分别标记5-溴脱氧尿苷(BrdU)阳性细胞和微管相关蛋白(DCX)阳性细胞,显微镜下观察BrdU与DCX阳性细胞的表达。结果:双根清脑颗粒中、低剂量组、脑复康组大鼠皮质区与SGZ区BrdU阳性细胞数均明显增加(P<0.05);双根清脑颗粒中、低剂量组SGZ区BrdU阳性细胞比脑复康组增加更为显著(P<0.01)。假手术组以及双根清脑颗粒中、低剂量组大鼠SGZ区DCX阳性细胞数明显增加(P<0.01),高剂量组、脑复康组大鼠SGZ区DCX阳性细胞数亦显著增加(P<0.05);双根清脑颗粒中、低剂量组大鼠SGZ区DCX阳性细胞数比脑复康组明显增加(P<0.01)。结论:双根清脑颗粒可促进VD模型大鼠脑内NSCs的增殖与迁移。Objective：To observe the effect of Shuanggen Qingnao granules on proliferation and migration of neural stem cells（NSCs） in model of rats with vascular dementia and its treatment mechanism for vascular dementia（VD）.Method：Forty two SPF level SD rats were randomly divided into large,medium,low-dose of Shuanggen Qingnao granules groups,piracetam group,model group,sham operation group,the normal group（n = 6,each）.VD rat model was established by slowly injecting the same kinds of thrombotic emboli to the catheter of the external carotid artery.5-bromo-2-deoxyuriding（BrdU） positive cells and doublecortin（DCX） positive cells were respectively marked by immunohistochemical method and their expressions were observed by microscope.Result： Compared with model group,whether in cerebral cortex or hippocampus,BrdU positive cells of medium,low-dose of Shuanggen Qingnao granules groups and piracetam group were significantly increased（P 〈 0.05）;compared with piracetam group,BrdU positive cells of medium,low-dose of Shuanggen Qingnao granules groups in cerebral hippocampus were also significantly increased（P 〈 0.01）.DCX-positive cells in rats＇SGZ district of normal group,sham operation group and medium,low-dose of Shuanggen Qingnao granules groups were significantly increased（P 〈0.01）;compared with the model group,DCX-positive cells of large-dose of Shuanggen Qingnao granules group and piracetam group were also significantly increased（P 〈 0.05）;DCX-positive cells in rats＇SGZ district of medium,low-dose of Shuanggen Qingnao granules groups compared with the piracetam group were obviously increased（P 〈 0.01）.Conclusion：Shuanggen Qingnao granules can promote the proliferation and migration of NSCs in inner brain of VD rats.

关 键 词：双根清脑颗粒 血管性痴呆 神经干细胞 增殖 迁移 

分 类 号：R285.5[医药卫生—中药学]