重组人血小板活化因子乙酰水解酶保护小鼠脑缺血再灌注的研究  

Recombinant human platelet activating factor acetylhydrolase for protection of cerebral ischemia-reperfusion in mice

作　　者：吴宜娟[1,2,3,4,5] 王丽娟[1,2,3] 张雄[1,2,3] 代成波[1,2,3] 王硕[1,2,3] 马桂贤[1,2,3] 

机构地区：[1]广东省人民医院神经科 [2]广东省医学科学院 [3]广东省医学科学院神经科学研究所,广东广州510080 [4]南方医科大学,广东广州510515 [5]广州医学院第一附属医院,广东广州510182

出　　处：《中风与神经疾病杂志》2010年第10期868-870,共3页Journal of Apoplexy and Nervous Diseases

基　　金：广东省自然科学基金资助项目(8151008004000008)

摘　　要：目的观察重组人血小板活化因子乙酰水解酶(rPAF-AH)对小鼠脑缺血再灌注的保护作用。方法采用线栓法建立小鼠大脑中动脉缺血再灌注模型,尾静脉注射rPAF-AH预处理后观测模型小鼠神经行为学和脑梗死灶大小的改变,采用Westernblot研究MMP-2的表达及活性,同时与奥扎格雷钠注射液、金纳多注射液及假手术对照组比较。结果 rPAF-AH预处理明显降低脑梗死小鼠行为障碍的神经行为学评分和脑梗死灶的大小(P<0.01)。Westernblot分析结果证明,rPAF-AH预处理可降低脑缺血再灌注损伤所诱导的MMP-2蛋白的表达及其活性水平(P<0.01)。结论 rPAF-AH预处理对小鼠脑缺血再灌注损伤有一定的保护作用,这可能得益于其抑制了MMP-2蛋白的高表达及活性增高。Objective To observe the protection of recombinant human platelet activating factor acetylhydrolase （rPAF-AH）on cerebral ischemia-reperfusion mice.Methods The cerebral ischemia-reperfusion model was establishied with nylon thread to obstruct the middle artery in mice.After pretreatment with（rPAF-AH） and Ozagrel sodium injection and Extract of Ginkgo Biloba Leaves lnjection,neurological deficits scores,index of cerebral infarction were determined.MMP-2 level were measured by Western blot.Results rPAF-AH obviously reduced neurological deficits scores and index of cerebral infarction （P0.01）.Result of Western blot indicated that rPAF-AH could reduce the elevated levels of MMP-2 expression and activity induced by cerebral ischemia reperfusion.Conclusion The prophylactic treatment of rPAF-AH has protective effect against injury of cerebral ischemic reperfusion,which could be attributed to its property against MMP-2.

关 键 词：血小板活化因子乙酰水解酶 脑缺血再灌注 基质金属蛋白酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]