槟榔碱干预后的血管内皮细胞上清液对口腔颊黏膜成纤维细胞生物活性的影响  

Influence of supernatant from endothelial cells intervened by arecoline on bioactivity of fibroblast

作　　者：李明 彭解英[2] 庞丹琳[2] 尹晓敏[2] 

机构地区：[1]湖南省长沙市口腔医院,湖南长沙410005 [2]中南大学湘雅医院口腔科,湖南长沙410078

出　　处：《中国现代医学杂志》2010年第19期2941-2943,2949,共4页China Journal of Modern Medicine

基　　金：湖南省科技厅社会发展基金资助项目(No:02SSY3052)

摘　　要：目的比较不同浓度槟榔碱干预后的血管内皮细胞上清液(AECS)对正常人、OSF患者成纤维细胞(NM-FB、OSF-FB)增殖和胶原合成的影响。方法 0、30、60、90和120μg/mL槟榔碱干预血管内皮细胞(EC)24h,收集去除槟榔碱后EC在无血清培养液中的上清液AECS并干预FB。结果 AECS30、AECS60、AECS90较AECS0更能促进FB的增殖和胶原合成(P<0.05),但AECS120与AECS0无明显差别(P>0.05)。结论低浓度槟榔碱干预后的EC分泌功能改变促进FB的增殖和胶原合成能力增强,EC可能参与了OSF的形成。【Objectives】To compare the influence of supernatant from endothelial cells（ECs） intervened by arecoline（AECS） on the proliferation and collagen synthesis of fibroblasts fro normal people and oral submucous fibrosis（NM-FBs,OSF-FBs）.【Methods】The supernatants were collected from ECs which were intervened by 0,30,60,90 and 120 μg/mL arecoline for 24 h and then cultured in serum-free medium without arecoline.FBs were intervened by the above supernatants.【Results】AECS30,AECS60 and AECS90 were more powerful than AECS0 to promote the proliferation and collagen synthesis of FBs（P 〈0.05）.There was no significant difference between AECS120 and AECS0（P〉0.05）.【Conclusions】The secretion change of EC intervened by low concentration arecoline may promote proliferation and collagen synthesis of FBs.ECs may be involved in the development of OSF.

关 键 词：口腔黏膜下纤维性变 槟榔碱 成纤维细胞 血管内皮细胞 

分 类 号：R781.5[医药卫生—口腔医学]