Beagle犬静脉注射乳化异氟醚的急性毒性  

Acute toxicity of intravenous isoflurane in Beagles

作　　者：刘爱杰[1] 张文胜[1] 马汉祥[2] 杨小霖[3] 杨宗斌[4] 饶艳[5] 罗南富[1] 刘进[1] 

机构地区：[1]四川大学华西医院麻醉与危重病医学教研室,成都市610041 [2]宁夏医学院附属医院麻醉科 [3]川北医学院麻醉科 [4]利川市人民医院麻醉科 [5]武汉大学中南医院麻醉科

出　　处：《中华麻醉学杂志》2010年第7期772-773,共2页Chinese Journal of Anesthesiology

基　　金：基金项目：国家自然科学基金（30271259）

摘　　要：目的研究Beagle犬静脉注射乳化异氟醚的急性毒性。方法健康成年Beagle犬6只，雌雄各半，月龄6～8月，体重6～8k，设定乳化异氟醚注射液（120mg／m1）剂量序列为1．2、1．5、1．8、2．1、2．4、2．7、3．0、3．3、3．6、3．9ml／kg。用30％脂肪乳定容至d．0ml／kg，注射时间60s，以3．0ml／kg为初始剂量，采用间隔剂量给药的方法，注射后犬死亡，则下一只犬间隔一个低剂量给药，注射后犬未死亡，则下一只犬间隔一个高剂量给药，确定最大耐受剂量（MTD）和最低致死剂量，然后用这两个剂量之间的剂量[近似致死剂量（ALD）]给1只犬注射。观察毒性反应，死亡犬作大体解剖，体积、颜色、纹理改变的器官作组织病理学检查。结果Beagle犬静脉注射乳化异氟醚的ALD和MTD分别为252、216mg／kg，其急性毒性反应主要表现为BP降低，呼吸变浅、变慢，HR降低，出现室颤。死亡犬的大体解剖和各脏器组织病理学检查结果均未见异常。结论Beagle犬静脉注射乳化异氟醚的ALD和MTD分别为252、216mg／kg，其急性毒性反应主要表现为呼吸和循环功能抑制。Objective To investigate the acute toxicity of intravenous isoflurane in Beagles. Methods Six healthy adult Beagles of both sexes aged 6-8 months weighing 6-8 kg were used in this study. Isoflurane injeetio （120 mg/ml） in 30% lipid emulsion was injected intravenously. Femoral artery was cannulated for direct BP monitoring. ECG was continuously monitored. The maximal tolerance dose （MTD） and approximate lethal dose （ALD） were determined by up-and-down technique. The initial dose was 3.0 ml/kg. The close was decreased/ increased by 0.3 ml/kg if the previous animal died/survived. The survived dogs were observed for 2 weeks. Autopsy and histopathologieal examination were performed on all dead Beagles. Results The ALl） and MTD of intravenous isoflurane were 252 and 216 mg/kg. Autopsy and histopathological examination did not show any abnormality. Conclusion Cardiopuhnonary depression is the main manifestation of the acute toxicity of intravenous isoflurane in Beagles.

关 键 词：异氟醚 急性毒性实验 最大耐受剂量 

分 类 号：R614[医药卫生—麻醉学]