吗啡对人胃癌MGC-803细胞p53mRNA和E2F-1mRNA表达的影响  

Effect of morphine on expression of p53 mRNA and E2F-1 mRNA in human gastric carcinoma cell line MGC-803

作　　者：覃怡[1] 唐小曼[1] 廖淳杰[1] 谢玉波[1] 

机构地区：[1]广西医科大学第一附属医院麻醉科,南宁市530021

出　　处：《中华麻醉学杂志》2010年第7期840-842,共3页Chinese Journal of Anesthesiology

摘　　要：目的探讨吗啡对人胃癌MGC-803细胞p53mRNA和E217-1mRNA表达的影响。方法人胃癌MGC．803细胞以1×10 3／ml或2×10 5／ml密度接种于6孔培养板中，1ml／孔，随机分为2组（n=18），正常对照组（c组）不作任何处理，吗啡组（M组）加入吗啡，使其终浓度为10umol／L。于吗啡孵育7d时应用克隆形成实验测定细胞增殖情况，于吗啡孵育24h时测定细胞中p53mRNA、E2F-1mRNA的表达情况，并采用透射电子显微镜观察细胞超微结构。结果与C组比较，M组克隆形成率降低，p53mRNA表达上调，E2F-1mRNA表达下调（P〈0．05）。c组细胞核膜完整、核仁及染色质清晰，M组细胞核膜破裂、核仁碎裂，并出现凋亡小体。结论吗啡可上调人胃癌MGC-803细胞p53基因表达、下调E2F—1基因表达，从而抑制细胞增殖，促进细胞凋亡。Objective To investigate the effect of morphine on the expression of p53 mRNA and E2F-1 mRNA in human gastric carcinoma cell line MGC-803. Methods The human gastric cancer cell line MGC- 803 was purchased from Cell Biology Research Institute, Chinese Academy of Sciences, and cultured in DMEM liquid culture medium. The cells were seeded in 6-well plates （1 × 10 3/ml or 2 × 10 5/ml, 1 ml/well） and divided into2 groups （n = 18 wells each）： group I normal control （group C）; group II was exposed to 10 umol/L morphine （group M）. The proliferation of the cells was determined by colony formation assay at 7 day of incubation with morphine. The expression of p53 mRNA and E2F-1 mRNA was detected and the ulrastructure of the cells examined with transmission electron microscope after being incubated with morphine for 24 h. Results The proliferation of the ceils and E2F-1 mRNA expression were significantly lower and p53 mRNA expression was significantly higher in group M than in group C （ P 〈 0.05）. The nuclear cvelope was intact and the nucleolus and chromosomes were clearly visible in group C, while in group M fragmentation of nuclear envelope and nucleolus and apoptotic bodies were observed. Conclusion Morphine can inhibit the proliferation of the cells and accelerate the cell apoptosis through up-regulating the expression of p53 gene and down-regulating the expression of E2F-1 gene in human gastric carcinoma cell line MGC-803.

关 键 词：吗啡 细胞系 肿瘤 肿瘤抑制蛋白质P53 E2F-1转录因子 RNA 信使 

分 类 号：R735.2[医药卫生—肿瘤]