高温海藻糖合酶产生菌SN02004-01发酵产酶条件研究  

Study on optimization of fermentation conditions with thermostable trehalose synthetase-Producing Bacterium SN 02004-01

作　　者：王俊峰[1] 周启扉[1] 刘大伟[1] 刘志健[1] 

机构地区：[1]黑龙江农业工程职业学院,哈尔滨150088

出　　处：《食品科技》2010年第11期11-15,共5页Food Science and Technology

基　　金：辽宁省自然科学基金项目(2040515)

摘　　要：采用摇瓶发酵法研究了Bacillus sp.SN02004-01培养特性和最佳发酵产酶条件,优化培养基组成为:麦芽糖1.0%、蛋白胨0.5%、酵母膏0.5%、硝酸钠0.075%、硫酸镁0.025%、氯化钙0.01%、磷酸氢二钾0.01%;其最佳的发酵条件为:接种9 h种龄种子、接种量6%、最佳发酵温度为65℃、最佳发酵pH为7.0、发酵终点为24 h。The optimal fermentation conditions of Bacterium Bacillus sp.SN02004-01 producing thermostable trehalose synthetase were investigated in this paper.The optimal culture medium were as followed： maltose 1%,peptone 0.5%,yeast extraction 0.5%,NaNO3 0.075%,MgSO4 0.025%,CaCl2 0.01% and K2HPO4 0.01%.The optimal environmental conditions were as followedi：noculumage 9 h,spores 6%,initial pH 7.0,fermention temperature 65 ℃ and fermention time 24 h.

关 键 词：海藻糖 高温海藻糖合酶 发酵条件优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]