绳状青霉菌发酵产右旋糖酐酶的条件研究  

Optimizing the Fermentation Conditions of Penicillium funiculosum for Producing Dextranase

作　　者：朱慧霞[1] 王雅洁[2] 邓胜松[1] 姚日生[1] 张洪斌[1] 

机构地区：[1]合肥工业大学化学工程学院,农产品生物化工教育部工程研究中心,安徽合肥230009 [2]安徽医学高等专科学校药学系,安徽合肥230601

出　　处：《食品科学》2010年第19期288-291,共4页Food Science

基　　金：安徽高校省级自然科学研究重点项目(KJ2008A067)

摘　　要：研究绳状青霉菌(Penicillium funiculosum)发酵培养产右旋糖酐酶的条件。研究碳源、氮源对绳状青霉菌产右旋糖酐酶的影响,结果表明最适合发酵产酶的碳源、氮源分别是右旋糖酐和蛋白胨。采用正交试验对绳状青霉菌产右旋糖酐酶的发酵条件进行优化,结果表明最适条件为:发酵瓶装液量为80mL/250mL,发酵培养基的初始pH值为5.5,培养温度为28℃,在该条件下,右旋糖酐酶的酶活力为18.963U/mL。This study aimed at investigating the optimal fermentation conditions of Penicillium funiculosum for producing dextranase. The optimal carbon and nitrogen sources were dextran and peptone,respectively. The orthogonal array optimization showed that the optimal fermentation conditions of Penicillium funiculosum were as follows：the volume of the medium contained in a 250 mL Erlenmeyer flask 80 mL; medium initial pH 5.5; and fermentation temperature 28 ℃,and the resultant dextranase was 18.963 U/mL under these conditions.

关 键 词：右旋糖酐酶 绳状青霉菌 正交试验 

分 类 号：Q936[生物学—微生物学]