一株高效好氧反硝化菌的分离及特性  

Isolation and Characterization of a High Efficiency Aerobic Denitrifier

作　　者：杨俊忠[1] 倪砚[1] 许尚营[1] 徐可瀚[1] 刘义[1] 曾丽霞[1] 刘德立[1] 

机构地区：[1]f华中师范大学生命科学学院湖北省遗传调控与整合生物学重点实验室,湖北武汉430079

出　　处：《微生物学通报》2010年第11期1594-1599,共6页Microbiology China

基　　金：国家自然科学基金项目(No.30170011,30771429);教育部博士点基金项目(No.20060511002);湖北省科技攻关计划项目(No.2007AA201C50,2007AA301C26)

摘　　要：利用富集培养的方法从南昌市郊某养鱼塘采样分离出22株反硝化细菌,其中8株反硝化率较高,从中选择一株效果最好的作为研究对象,命名为HS-N62,对其生长特性进行了深入研究。结果表明:硝酸盐氮初始浓度为140mg/L,菌株HS-N62在12h内对硝酸盐氮的去除率可达96%,而且没有亚硝酸盐氮的积累。该菌最适生长温度范围为30°C-37°C,最适生长pH范围6.0-8.0,最适C/N比为10:1,并能利用多种碳源生长。运用正交试验探讨了该菌株最适的反硝化条件。反硝化菌株HS-N62还具有较好的除磷能力,12h除磷率达到67.7%(初始磷酸盐浓度57mg/L)。通过形态学特性和生理生化分析以及16S rRNA基因序列分析,菌株HS-N62与Pseudomonas sp.亲缘关系最为接近,相似性达99%,初步鉴定该菌为假单胞菌属(Pseudomonas sp.)。Twenty two bacterial strains with a capacity of denitrification were isolated from fish pond,of which eight strains have the high rate of denitrification while one strain named HS-N62 has the strongest effect of denitrification.The results showed that the denitrifying rate of strain HS-N62 reached 96% in 12 hours with initial nitrate nitrogen concentration of 140 mg/L and no nitrite nitrogen accumulation.The growth rate and characteristics of HS-N62 were further studied,and the results showed that the optimum growth temperature ranged from 30°C to 37°C,pH value from 6.0 to 8.0 and the optimal C/N ratio 10：1.Meanwhile strain HS-N62 could utilize a variety of carbon sources for growth.The most suitable denitrification condition for strain HS-N62 was confirmed by using a series of the orthogonal test.This denitrifying strain HS-N62 also has good ability of phosphorus removal,and the removal rate within 12 hours was up to 67.7%（at initial phosphate concentration 57 mg/L）.Strain HS-N62 showed 99% 16S rRNA gene sequence similarity to many Pseudomonas strains.Based on the morphological characteristics,physiological and biochemical characteristics,the strain HS-N62 was identified as Pseudomonas sp.

关 键 词：好氧反硝化 分离鉴定 特性研究 

分 类 号：X172[环境科学与工程—环境科学]