滇南红厚壳叶中木栓内酯的分离、结构鉴定及诱导干细胞增殖作用  

Separation,structural characterization and inducing proliferation activity of Friedelin-3,4-lacton from Calophyllum polyanthum

作　　者：王辉[1] 熊志勇[2] 陈泽宇[3] 曾和平[1] 

机构地区：[1]华南师范大学化学与环境学院,广东广州510006 [2]北京理工大学珠海学院化工与材料学院,广东珠海519085 [3]云南西双版纳出入境检验检疫局,云南景洪666100

出　　处：《云南大学学报（自然科学版）》2010年第6期690-694,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：广东省科技计划资助项目(2004B33001080);广州市科技计划资助项目(2005J1-C0131)

摘　　要：滇南红厚壳叶经甲醇浸提并浓缩得浸膏,提取浸膏再用石油醚萃取.石油醚溶解部位经硅胶柱层析,石油醚-乙酸乙酯作为洗脱剂梯度洗脱,获得4个化合物,其中在石油醚-乙酸乙酯体积比为9:1的洗脱条件下首次从该植物中分离得到一种白色柱状晶体.经IR,MS,1HMNR和13CMNR以及X射线单晶衍射对分离化合物进行结构表征,确定所获化合物1为木栓内酯.采用MTT法研究了木栓内酯诱导小鼠骨髓间质干细胞的增殖活性,显示出一定的促进作用.The methanol extract of the dried leaf of Calophyllum polyanthum was partitioned successively with petroleum ether and ethyl acetate.Petroleum ether fraction was gradient eluted over silica gel column using 9：1 petroleum ether-ethyl acetate as eluant to give a rod crystal and three known compounds.The rod crystal was characterized by IR,1H NMR,13C NMR,MS and X-ray single crystal diffraction.The results showed that the crystal was friedelin-3,4-lactone,and the molecular formula was C30H50O2.Its bioactivity was measured by MTT assay.The results proved that triterpenoid lactone has a promotion effect on the inducing proliferation of rat mesenchymal stem cells（rMSCs）.

关 键 词：滇南红厚壳 木栓内酯 结构表征 诱导增殖 

分 类 号：Q946[生物学—植物学] Q949.758.3