高效液相色谱柱前衍生法测定金银花中氨基酸的含量  

Determination of Amino Acids in Flos Lonicerae by HPLC Coupled With Pre-column Derivatization

作　　者：王薇[1] 周正礼[2] 李健 李峰[2] 

机构地区：[1]陕西中医学院,陕西咸阳7120462 [2]山东中医药大学药学院,山东济南250355 [3]山东省中医医院药剂科,山东济南250014

出　　处：《辽宁中医杂志》2010年第11期2212-2213,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512601)

摘　　要：目的:建立一种用异硫氰酸苯酯柱前衍生的高效液相色谱方法测定金银花中17种氨基酸含量的方法,为金银花药性物质基础研究提供实验依据。方法:水解金银花总蛋白,以异硫氰酸苯酯进行柱前衍生,用HPLC测定金银花中氨基酸峰面积并计算其含量。结果:各种氨基酸均能达到基线分离。结论:高效液相色谱柱前衍生法可作为金银花药材中初生成分的测定手段之一。Objective：To establish a method for determination of amino acids Flos Lonicerae and provide experimental basis for the physical basis of properties. Methods：The total proteins were separated,after that,hydrolyzed the total proteins,followed by pro-column derivation with Phenylisothiocyanate（ PITC） and determined peak area of every amino acid by RP-HPLC method,at last calculated the cotent of every amino acid. Results：Using HPLC every amino acid can be separated well. Conclusion：This meth-od is a good way in the determination of amino acid content.

关 键 词：金银花 氨基酸 高效液相色谱 药性 

分 类 号：R284.1[医药卫生—中药学]