栓线法大鼠局灶性脑缺血模型的建立与改进  

Establishment and Improvement－A Rat's Model of Focal CerebralIschemia (FCI) by Nylon Suture Embolization

作　　者：吴莹[1] 陈文荣[2] 吴春泽[1] 陈玲[1] 黄 荷 刘兴材[1] 

机构地区：[1]汕头大学医学院第二附属医院神经内科,汕头515061 [2]汕头大学医学院第二附属医院神经外科,汕头515061

出　　处：《汕头大学医学院学报》1999年第1期33-35,共3页Journal of Shantou University Medical College

摘　　要：选择大脑中动脉闭塞法（MCAO）来建立大鼠局灶性脑缺血（FCI）模型。我们将惯用的栓线法进行如下改进：①不分离和结扎翼腭动脉（PPA）不用动脉夹夹闭颈内动脉，按Zealonga5分制对术后大鼠进行神经病学评分，除6只假手术外，30只大鼠计分为2－3分，表明模型成功。TFC染色能清楚显示梗塞范围，HE染色后光镜下见梗塞区有典型缺血性变化如细胞肿胀、变性、坏死。改进法模型操作方便，省时间，成功率几乎达100％。objective: To establish a simple and convenient rat's model of focal cerebral ischemla. 'me conventionalnylon sutUre echlization (WSE) for griddle cerebral artery exclusion (MCAO) Icas improved as fbllot\-s: (D thepteryopalatine artery (PPA) was not dissected and ligated: O the internal carotid aloe1~' (ICA ) Icas not clipped.Result: Showing that 30 rats with cerebral ischemia Ivere scored 2 or 3 according to neulnlogic sconng, and the typicalischendc change was found in the infarct areas. Conclusion: This impro17ed method provides a net'' simple andconvenient model for stud},ing focal cerebral ischennia Ividl very high successful rate of nearly loo per cent.

关 键 词：脑缺血 动物模型 大鼠 栓线法 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]