锂盐对大鼠腭中缝扩张成骨的影响  

Effect of Lithium on the Osteogenesis during Mid Palatal Suture Expansion of Rats

作　　者：徐疾[1] 周洁[1] 唐国华[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔正畸科.上海市口腔医学重点实验室,上海200011

出　　处：《口腔医学研究》2010年第5期620-623,共4页Journal of Oral Science Research

基　　金：上海市科学技术委员会基金(编号:8DZ2271100;10QH1401600);上海市重点学科建设项目资助(编号:S30206);上海市教育委员会基金(编号:09YZ75)

摘　　要：目的:观察锂盐对腭中缝扩张后新骨形成的影响。方法:使用扩大簧对Wistar大鼠作快速扩弓,腭中缝扩大3d后每日给予LiCl灌胃,对照组使用NaCl。通过甲苯胺蓝染色定量分析新骨的形成,免疫组化染色检测β-catenin的表达。结果:腭中缝扩大后第3天开始有新骨形成,应用LiCl后新骨边缘成骨细胞表达β-catenin增加。半定量分析显示实验组7d新骨形成量显著增加,是对照组的1.36倍(P<0.05)。结论:锂盐能够通过激活β-catenin信号,促进大鼠腭中缝扩大后的新骨形成。Objective：To evaluate the role of lithium in the osteogenesis of mid palatal expansion of rats.Methods：Wistar rats were subjected to rapid palatal suture expansion with a helix spring on the maxilla.The rats in experimental groups were gavage-fed LiCl solution daily 3 days after the expansion,while the rats in control groups were fed saline.The amount of new bone formation was quantified by toluidine blue staining.β-catenin was detected through immunohistochemical staining.Results：The new bone deposition was observed in the mid palatal suture 3 days after expansion.Higher level of the expression of β-catenin was found in the osteoblasts on the boundary of the new bone after LiCl application.Semi-quantitative analysis showed that the new bone volume of the LiCl treated rats was 1.36 times of the NaCl treated animals 7 days after the expansion（P〈0.05）.Conclusions：Lithium treatment promotes the osteogenesis in the mid palatal suture expansion in rats through the activation of β-catenin signaling.

关 键 词：腭中缝 牵张力 骨形成 锂盐 Β-CATENIN 

分 类 号：R783.5[医药卫生—口腔医学]