p53介入治疗对VX2兔肝癌侵袭转移特性的影响  

Effect of interventional treatment with p53 on the invasion and metastasis of VX2 liver tumor in experimental rabbits

作　　者：李彩霞[1] 冯艳[1] 谷涛[1] 李春海[1] 

机构地区：[1]济南山东大学齐鲁医院放射科,250012

出　　处：《介入放射学杂志》2010年第10期800-804,共5页Journal of Interventional Radiology

基　　金：山东省卫生厅资助项目(2001CAICJA12);山东省自然科学基金Y2007C004

摘　　要：目的探讨p53介入治疗对VX2兔肝癌侵袭转移的影响。方法开腹方法将VX2瘤细胞分别植入48只新西兰大白兔的肝左叶,建立VX2兔肝癌模型,随机分为4组(12只/组),分别进行肝动脉插管介入治疗,对照组注入生理盐水、A组、B组、C组分别注入水化碘油、Ad-p53、Ad-p53+水化碘油。术后1周处死肿瘤兔,免疫组化方法测定基质金属蛋白酶-2(MMP-2)、增殖细胞核抗原(PCNA)、血管内皮生长因子(VEGF)表达。结果实验组(A,B,C组)观测到肿瘤生长受到明显抑制,与对照组比较差异有统计学意义(P<0.05)。单纯碘油栓塞后,肿瘤区MMP-2、PCNA表达略有降低,VEGF表达略有升高,与对照组比较差异均无统计学意义(P>0.05);Ad-p53组与Ad-p53+水化碘油组的MMP-2、PCNA及VEGF的表达阳性率降低,与对照组相比差异有统计学意义(P<0.05);转移组的MMP-2、PCNA及VEGF的表达阳性率均高于无转移组(P<0.05);MMP-2与VEGF及PCNA之间均存在相关性(P<0.05)。结论 MMP-2、VEGF及PCNA的增高预示着肿瘤高转移、高增殖能力,肿瘤血管高形成能力。Ad-p53及Ad-p53+碘油介入治疗可抑制肿瘤的生长,抑制肿瘤新生血管形成,减少转移。Objective To investigate the effect of interventional treatment with p53 on the invasion and metastasis of VX2 liver tumor in experimental rabbits.Methods VX2 carcinoma cells were surgically implanted into the left hepatic lobe in 48 New Zealand white rabbits,and the rabbit hepatic carcinoma models were thus established.The rabbits were randomly divided into 4 groups with 12 rabbits in each group.After hepatic arterial catheterization was completed physiological saline（control group）,Lipiodol（Group A）,Ad-p53（Group B） and Lipiodol＋Ad-p53（Group C） were respectively infused into the rabbits of four groups via common hepatic artery.One week after the procedure the rabbits were sacrificed and the livers were removed for the determination of matrix metalloprotein-2（MMP-2）,proliferating cell nuclear antigen（PCNA） and vascular endothelial growth factor（VEGF） of the tumor with immunohistochemistry technique.Results The tumor growth in study groups（group A,B and C） was markedly suppressed,which was significantly different in comparison with that in control group（P 0.05）.In group A the positive rates of MMP-2,PCNA were slightly lower,while the positive rate of VEGF was slightly higher,than that in control group although the difference was of no significance（P 0.05）.The positive rates of MMP-2,PCNA and VEGF in group B and C were significantly lower than those in control group（P 0.05）.The positive rates of MMP-2,PCNA and VEGF of the rabbits with metastasis were markedly higher than those without metastasis（P 0.05）.MMP-2 bore a certain relationship with VEGF and PCNA（P 0.05）.Conclusion The increase of the positive rates of MMP-2,PCNA and VEGF indicates that the tumor possesses higher possibility for developing metastasis,proliferation and vascular formation.The interventional treatment with Adp53 or Lipiodol＋Ad-p53 can inhibit the growth,metastasis and vascular formation of VX2 liver tumor in experimental rabbits.（J Intervent Radiol,2010,19：800-804）

关 键 词：P53 介入治疗 兔 VX2肝癌 基质金属蛋白酶-2 增殖细胞核抗原 血管内皮生长因子 

分 类 号：R735.7[医药卫生—肿瘤]