不同类型乳腺组织中CD44^+/CD24^-细胞的数量与分布  

Number and distribution of CD44^+/CD24^- cells in different type of breast tissues

作　　者：吕新全[1] 金湘东[2] 吕会艳[3] 徐珂佳[3] 索振河[3] 李惠翔[1] 

机构地区：[1]郑州大学第一附属医院病理科/河南省肿瘤病理重点实验室,郑州450052 [2]郑州铁路职业技术学院医学技术系,郑州450052 [3]郑州大学第一附属医院肿瘤科,郑州450052

出　　处：《临床与实验病理学杂志》2010年第5期536-539,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河南省基础与前沿技术研究计划项目(08230045460)

摘　　要：目的检测CD44+/CD24-细胞在乳腺正常组织及良恶性肿瘤中的数量与分布特点,探讨其在乳腺癌发生、发展中的作用。方法采用免疫组化SP双染法,检测了30例乳腺正常组织(normal tissue,NT),30例乳腺纤维腺瘤(fibroadenoma,FA),60例乳腺浸润性导管癌(invasive ductal carcinoma,IDC)中的CD44及CD24的共表达情况。结果在乳腺NT及FA中,可见CD44+/CD24+,CD44+/CD24-,CD44-/CD24-3种表型,而在IDC中,除上述3种表型外,尚可见CD44-/CD24+表型。从NT,FA到IDC,CD44+/CD24-细胞的阴性率(-,阳性率<1%)依次降低(40.0%→36.7%→35.0%);而强阳性率(▲,阳性率>60%)依次升高(0.0→6.7%→21.7%)。结论 CD44+/CD24-可能作为一种阶段性祖细胞标记,参与了正常乳腺的分化及乳腺肿瘤的发展过程。Purpose To examine the quantity and the distribution of CD44＋/CD24-cells in breast normal tissue（NT）,fibroadenoma（FA）and invasive ductal carcinoma（IDC） and to investigate the function of the cells in breast carcinogenesis.Methods Double staining immunohistochemistry of CD44 and CD24 was performed on 30 cases of NTs,30 FAs,and 60 IDCs.Results In normal breast tissue and FAs,three phenotypes were observed including CD44＋/CD24＋,CD44＋/CD24-,and CD44-/CD24-.In the IDCs,CD44-/CD24＋ was detected beside the three aforementioned phenotypes.The proportions with lowest score（-,incidence 1%）of CD44＋/CD24-were decreased（40.0%→36.7%→35.0%）,but the proportions with highest score（▲,incidence 60%）of CD44＋/CD24-were increased（0.0→6.7%→21.7%）from normal breast tissue,FAs to IDCs.Conclusions The CD44＋/CD24-may be a marker for certain progenitor cells,which are involved in the differentiation of normal breast and the pathogenesis of breast tumors.

关 键 词：乳腺肿瘤 CD44 CD24 干细胞 

分 类 号：R737.902.2[医药卫生—肿瘤]