Ouabain诱导建立大鼠弱精子症模型  

Study of Asthenospermia Animal Model Induced by Ouabain in Rat

作　　者：娄欢[1] 万艳[1] 沈小力[1] 苏萍[1] 

机构地区：[1]华中科技大学同济医学院计划生育研究所,武汉430030

出　　处：《生殖与避孕》2010年第10期653-658,共6页Reproduction and Contraception

摘　　要：目的:建立SD大鼠腹腔注射Ouabain诱导的弱精子症动物模型。方法:健康性成熟SD雄性大鼠40只,随机分为对照组(生理盐水,n=10),低剂量实验组(Ouabain 12.5μg/kg,n=15),高剂量实验组(Ouabain 25μg/kg,n=15),各组大鼠连续每日腹腔注射相应试剂,qd×30 d,观察各组附睾精子活力及活动率的变化,同时观察本实验剂量范围的Ouabain对大鼠生殖系统及全身可能的毒性反应。结果:与对照组相比,实验组大鼠精子数量无明显变化,但精子活力及活动率显著下降。高剂量及低剂量Ouabain组连续给药30 d后大鼠附睾精子活力分别为48.27%和44.73%,与对照组(73.33%)相比有显著性差异(P<0.01),且高、低剂量组间差异显著(P<0.01);高、低剂量组大鼠精子活动率分别为52.26%和49.95%,与对照组(78.70%)相比有显著性差异(P<0.01),高、低剂量组间亦有统计学差异(P<0.01)。本实验剂量范围内,Ouabain对大鼠生殖器官及全身重要脏器未显示明显的毒性反应。结论:Ouabain经腹腔注射给药30 d能成功诱导建立SD大鼠弱精子症模型的,且未见明显的生殖系统和全身毒性反应。Objective： To estabish asthenosrermia animal model in SD rats by injecting Ouabain.Methods： Forty adult male SD rats were randomly divided into control group（n=10,saline）,low dose experimental group（n=15,Ouabain 12.5 μg/kg） and high dose experimental group（n=15,Ouabain 25 μg /kg）.After intraperitoneal injecting for 30 d,each group was observed for their epididymal sperm motility,activate rate and the possible toxicity of reproductive system and the body.Results： The high and low dose groups’ epididymal sperm motility was respectively 48.27% and 44.73%;epididymal sperm activate rate respectively decrease to 52.26% and 49.95% compared with control group（73.33%,78.70%）,P0.01.The differences among the sperm activate rate of these three group were significant（P0.01） too.Ouabain on reproductive organs and the body of important organs in rats did not show obvious toxicity in test dose range.Conclusion： Asthenospermia SD rat model without significant reproductive and systemic toxicity was successfully established by intraperitoneal injecting Oua-bain for 30 d.

关 键 词：OUABAIN 弱精子症 精子活力 精子活动率 

分 类 号：R698.2[医药卫生—泌尿科学] R-332[医药卫生—外科学]