Simultaneous determination of five bioactive phenolic acids in Salvia yunnanensis and Salvia miltiorrhiza by HPLC  

HPLC法同时测定滇丹参和丹参中5个活性酚酸的含量(英文)

作　　者：张正付[1,2] 陈鸿珊[1] 李卓荣[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050 [2]北京大学化学与分子工程学院,北京100182

出　　处：《Journal of Chinese Pharmaceutical Sciences》2010年第4期271-278,共8页中国药学（英文版）

基　　金：National High-Tech R & D Program(863 Program, Grant No.2004AA2Z3342)

摘　　要：A reverse-phase HPLC method was developed for the simultaneous separation and determination of five bioactive phenolic acids,yunnaneic acid E,rosmarinic acid,lithospermic acid,salvianolic acid B and salvianolic acid A in eight different samples of Salvia yunnanensis collected in Yunnan Province.For comparison,the sample of Salvia miltiorrhiza was included. All the samples were extracted for 60 min with 50%methanol in an ultrasonic bath.The optimal separation was achieved on a YMC-Pack Pro C18 column,with a gradient of 0.1%（v/v） phosphoric acid and acetonitrile,at a flow rate of 1.0 mL/min and at a detection wavelength of 280 nm.The separation was obtained within 65 min for five bioactive phenolic acids.All calibration curves showed good linearity（r^2〉0.999） within test ranges.The relative standard deviation of the method was less than 5%for intra- and inter-day assays.The mean recovery of the method was in the range from 97%to 104%,with RSD less than 5%.This assay was successfully applied to the quantitative determination of five bioactive phenolic acids in nine resource samples. The results showed that the developed HPLC assay was suitable for the quality control of S.yunnanensis and it can be used to differentiate S.yunnanensis from S.miltiorrhiza.为同时测定不同产地滇丹参和丹参中5个具有抗HIV活性酚酸类化学成分(云南酚酸E,迷迭香酸,紫草酸,丹酚酸B和丹酚酸A)的含量,本文建立了RP-HPLC梯度色谱法。色谱柱:YMC-Pack Pro C_(18)(250 mm×4.6 mm,5μm)柱;流动相:0.1%(v/v)磷酸和乙腈,梯度洗脱;检测波长:280 nm;流速:1.0 mL/min。结果表明:所有这五个成分,在测试范围内,均呈良好的线性关系(r^2>0.999),日内和日间精密度RSD均小于5%,样品的平均加样回收率均在97%～104%范围内,RSD均小于5%。该法测定样品快速准确,重现性好,能用于滇丹参的质量控制,并可鉴别滇丹参和丹参药材。

关 键 词：Salvia yunnanensis Salvia miltiorrhiza Phenolic acids HPLC Quantitative determination 

分 类 号：R284.1[医药卫生—中药学]