猪IgGⅡB类Fc受体基因剪接异构体的克隆及序列分析  

Cloning and sequence analysis of porcine Fc gamma receptor IIB's transcript variant

作　　者：刘玉松[1] 夏平安[1] 刘肖萍[1] 张志远[1] 刘明莉[1] 王中明[1] 段二珍[1] 卢晓艳[1] 崔保安[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业大学学报》2010年第5期542-548,552,共8页Journal of Henan Agricultural University

基　　金：河南省重点攻关项目(92102110018)

摘　　要：根据GenBank中猪IgGⅡB类Fc受体(swFcγRⅡB)cDNA基因序列,利用Prim er软件设计合成了1对特异性引物,应用RT-PCR技术,从猪外周血白细胞cDNA中扩增swFcγRⅡB基因,将扩增的基因片段插入pTG19-T载体进行测序,利用DNAStar Protean软件对基因的氨基酸序列潜在抗原表位进行预测.结果表明,克隆的swFcγRⅡB基因序列长度为951 bp,编码316个氨基酸,与已发表序列的核苷酸与氨基酸序列同源性分别为99.3%和98.3%,其胞内区多出19个氨基酸的插入,其氨基酸序列至少存在3个潜在优势抗原表位的区域.swFcγRⅡB基因存在亚类,所克隆基因是swFcγRⅡB基因的1种RNA剪接异构体,预示了swFcγRⅡB基因至少存在2种RNA剪接异构体.According to the cDNA sequence of the porcine Fc gamma receptor IIB（swFcγRⅡB） in the GeneBank,a pair of primers were designed using primer.The gene of swFcγRⅡB was amplificated from porcine peripheral white blood cells using RT-PCR.Amplificated gene fragment was inseted into pTG19-T vector to sequencing.The putative epitope of the swFcγRⅡB＇s amino acid sequence was predicted by DNAStar Protean.According to the sequence analysis,the swFcγRⅡB＇s cloning gene fragment is 951 bp,and encodes 316 amino acids.The homology of nucleotide and amino acid sequence between FJ608551 and DQ026064 is 99.3% and 98.3%.Its intracellular region is augmented 19 amino acids compared with the publicated sequence.Its amino acid sequence exists more than 3 putative dominant antigen epitopes.The porcine Fc gamma receptor IIB exists subgroup,the gene we got is one kind of RNA transcript variant of the porcine Fc gamma receptor IIB.It can be predicted that there exist at teast two kinds of RNA transcript variant of the porcine Fc gamma receptor IIB.

关 键 词：猪 FcγRⅡB 抑制性受体 序列分析 抗原表位 

分 类 号：S852.2[农业科学—基础兽医学]