白血病患者白细胞糖皮质激素受体及其mRNA检测结果的比较  

Leukocyte glucocorticoid receptor and its mRNA levels in leukemia patients

作　　者：董群生[1,2] 孟沛霖[1,2] 闵碧荷[1,2] 童书鹏[1,2] 徐仁宝[1,2] 宋亮年[1,2] 卢健[1,2] 

机构地区：[1]第二军医大学附属长海医院血液科 [2]第二军医大学病理生理教研室

出　　处：《中华血液学杂志》1999年第5期252-254,共3页Chinese Journal of Hematology

摘　　要：目的对非霍奇金淋巴瘤细胞性白血病（ＮＨＬＣＬ）与急性淋巴细胞白血病（ＡＬＬ）进行鉴别诊断。方法采用放射配体结合分析法和以人糖皮质激素受体（ＧＲ）ｃＤＮＡ为探针的分子杂交技术对２２例ＮＨＬＣＬ、１８例ＡＬＬ、１０例慢性淋巴细胞白血病（ＣＬＬ）和６名健康人白细胞ＧＲ及ＧＲｍＲＮＡ进行了对比检测。结果ＮＨＬＣＬ和ＡＬＬ两类白血病患者的ＧＲ及ＧＲｍＲＮＡ水平比较，差异非常显著（Ｐ＜０．０１）；ＮＨＬＣＬ和ＣＬＬ两类白血病的ＧＲ及ＧＲｍＲＮＡ水平比较，差异不显著（Ｐ＞０．０５）。结论ＮＨＬＣＬ和ＡＬＬ两类白血病ＧＲ及ＧＲｍＲＮＡ水平的差异，对两者的鉴别诊断有重要的参考意义，两类白血病ＧＲｍＲＮＡ表达水平不同可能与细胞恶性增殖发生在不同分化阶段有关，确切机制有待进一步研究。Objective To explore factors for differentiating nonHodgkin's lymphoma cell leukemia (NHLCL) and acute lymphoblastic leukemia (ALL). Methods Leukocyte glucocorticoid receptor (GR) and GR mRNA levels were measured in 22 NHLCL, 18 ALL, 10 chronic lymphocytic leukemia (CLL) patients and 6 healthy subjects by using radioligand binding assay and molecular hybridization using GR cDNA as the probe. Results and Conclusions The leukocyte GR and GR mRNA levels were significantly different between NHLCL and ALL( P<0.01), and could be used as parameters for differential diagnosis of these two types of leukemia.

关 键 词：糖皮质激素受体 白血病 诊断 MRNA 

分 类 号：R733.7[医药卫生—肿瘤]