钆标记抗人肝癌单克隆抗体瘤内注射对荷瘤裸鼠的磁共振增强试验  

Experimental study on the contrast enhancement of MRI after intratumoral injection of gadolinium labelled antibodies against human hepatocellular carcinoma in the nude mouce model

作　　者：许乙凯[1,2,3] 刘杏元[1,2,3] 张嘉宁 俞志坚[1,2,3] 

机构地区：[1]广州第一军医大学南方医院影像中心 [2]第一军医大学动物所 [3]第一军医大学珠江医院放射科MRI室

出　　处：《放射学实践》1999年第2期106-107,共2页Radiologic Practice

摘　　要：目的：探讨小剂量瘤内注射钆标记抗人肝癌单克隆抗体的磁共振增强效应。方法：应用ＤＴＰＡ环酐双向偶合剂制出ＧｄＤＴＰＡＭｃＡｄｓ；实验组４只荷肝癌裸鼠直接瘤内注入３０μｇ单克隆抗体量的ＧｄＤＴＰＡＭｃＡｄｓ；对照组向瘤内注入３０μｇ单克隆抗体的生理盐水（ｎ＝２），１２ｈ后行Ｔ１加权像扫描。结果：实验组瘤内出现斑片状或不均匀强化，而对照组瘤体为均匀中等信号。结论：瘤内注射法能有效提高瘤靶内标记抗体的浓度，对改进ＭＲ免疫成像的敏感性有帮助。Purpose:To evaluate the possibility of MR targeting enhancement with local injection of a small dose of Gd labelled antihuman hepatocellular carcinoma McAbs(Hab 18).Methods:Gd DTPA was prepared by using CA DTPA to conjugate both Hab 18 antibodies and Gd 3+ . Of 6 nude mice bearing hepatocellular Carcinoma,4 cases were injected intratumorally with Gd DTPA McAbs at a dose of 30μg labelled antibody per mouse,while 2 were injected intratumorally with 30μg Hab 18 antibodies in normal saline as the control group. MR scans were performed 12 hours after the injection in all animals.Results :On T 1 weighted images,there were patchy or unhomogeneous enhancements in nodular lesions of experimental group,while no enhancement were noticed in the control group.Conclusion: Intratumoral injection could effectively raise the tissue concentration of the labelled McAbs in the nodular target.Therefore,it is a valuable and simple way for improving the sensitivity of MR immunoimaging.

关 键 词：肝癌 单克隆抗体 磁共振成象 

分 类 号：R735.705[医药卫生—肿瘤]