蜜蜂对接种球囊菌孢子幼虫清除行为的研究  

Removing behavior of adult honeybee to larvae innoculated by the spores of Ascosphaera apis

作　　者：李江红[1] 王海[1] 庄建斌[1] 郑志阳[1] 梁勤[1] 

机构地区：[1]福建农林大学蜂学学院,福州350002

出　　处：《中国蜂业》2010年第12期5-8,共4页Apiculture of China

基　　金：国家自然科学基金项目(30600454);国家蜂产业技术体系专项资金(NYCYTX-43)

摘　　要：为研究不同白垩病抗性蜜蜂蜂群对接种蜜蜂球囊菌孢子幼虫的清除行为。利用微量移液器对未患白垩病的意大利蜜蜂、患白垩病的意大利蜜蜂以及从未报道患病的中华蜜蜂的二日龄幼虫接种蜜蜂球囊菌孢子的水溶液,观察计算患病与否蜂群对接种幼虫的清除能力的差异。结果显示,未患病的意大利蜜蜂和中华蜜蜂对接种蜜蜂幼虫分别有65%和60%的清除率,而患病意大利蜜蜂则只有约22%的清除率。抗性蜂群对接种幼虫的清除主要发生在接种后的2～3天内和化蛹后,而患病蜂群在幼虫期则基本没有清除,只在蛹期有少量的清除。该结果说明,对染病幼虫的及时清除行为是蜜蜂抗白垩病的重要机制。To study the hygienic behavior of honeybee chalkbrood disease resistant and sensitive colony, the 2nd instar honeybee larvae from the resistant and sensitive honeybee colony （Apis mellifera and Apis cerana） were innoculated with the spores of Ascosphaera apis using the micropipettor, the innoculated honeybee larvae were then observed every 24 hours to caculate the removed larvae. The results showed that the removing rate of the calkbrood resistant honeybee colony were 65% for Apis mellifera, 60% for Apis cerana and 22% for the sensitive Apis mellifera colony. The removing time was mainly in the first 2 or 3 days after innoculation and the pupae time for the resistant of Apis mellifera and Apis cerana, and just the pupae time for the sensitive Apis mellifera colony. Thus the more effcient hygienic behavior was the main mechanism of honeybee chalkbrood resistance.

关 键 词：蜜蜂 球囊菌 孢子 接种 卫生行为 

分 类 号：S895.1[农业科学—特种经济动物饲养]