兔VX2肺肿瘤PET—CT与血管生成的相关性研究  

The correlation between PET-CT imaging and microvessed density in rabbit lung VX2 tumor model

作　　者：李亚军[1] 白人驹[1] 高硕[2] 李彦生[2] 刘磊[2] 贾薇[2] 蔡莉[2] 邢喜玲[2] 

机构地区：[1]天津医科大学总医院放射科,300052 [2]天津医科大学总医院PET-CT中心,300052

出　　处：《国际放射医学核医学杂志》2010年第3期139-142,共4页International Journal of Radiation Medicine and Nuclear Medicine

摘　　要：目的探讨^11C-胆碱和^18F-FDGPET—CT反映兔VX2肺肿瘤血管生成的效能。方法新西兰大白兔54只，右肺接种VX2肿瘤10—11d后，行^11C-胆碱和^18F-FDGPET—CT，测量肿瘤^11C-胆碱和^18F—FDG最大标准化摄取值（SUVmax）。同时，测量肿瘤标本大小，用免疫组化方法评价肿瘤微血管密度（MVD）。然后对肿瘤胆碱SUVmax。^18F、FDG SUVmax与肿瘤大小、MVD进行相关性分析。结果33只实验兔成功完成所有检查，得到VX2肺肿瘤的^11C-胆碱SUVmax平均值为4．02±3．07（1．4～12．2），对^18F—FDG的SUVmax平均值为5．70±3．45（1．0—13．0），肿瘤大小平均值为（1．68±1．61）cm^3（0．13—8．00cm^3）。高倍镜（200倍，0．739mm^2）视野下，肿瘤MVD平均值为（35．8±13．6）个（13—64个）。经统计学分析，^11C-胆碱SUVmax与肿瘤大小及MVD之间均不具有相关性；^18F-FDG SUVmax与MVD（r=0．525，P=0．002）之间存在正相关关系，与肿瘤大小（r=0．335，P=-0．057）之间呈临界正相关关系。结论^18F—FDG PET-CT可反映兔VX2肺肿瘤血管生成，^11C-胆碱PET-CT不能反映肿瘤血管生成。Objective To evaluate and compare the suitability of ^11C-choline and ^18F-FDG PET-CT for refleeting tumors angiogenesis. Methods Fifty-four New Zealand white rabbits whieh weighted 2.5-3.0 kg were used in the experiment. Under general anesthesia, a needle was transthoraeically inserted into the right lung, 0.5 ml viable VX2 tumor cell suspension was slowly injected through the needle to establish the model. ^11C-ehaline and ^18F-FDG PET-CT were performed after 10-11 d. The tumors SUVmax, were calculated. The sections were stained with hematoxylin and eosin, and immunostained for CD34. Assessment of mierovessel density（MVD） was performed by computer-assisted image analysis. The relationship ^11C-eholine SUVmax and ^18F-FDG SUVmax with tumor size and MVD were statistically analyzed. Results Thirty-three rabbits successfully completed all imaging examinations, ^11C-choline and ^18F-FDG differently accumulated in all lung VX2 tumors. The mean of HC-eholine SUVmax was 4.02＋3.07 （1.4-12.2）, and the mean of ^18F-FDG SUVmax was 5.70＋3.45 （1.0-13.0）. The mean size of tumor was （1.68＋1.61）cm^3 （0.13-8.00 cm^3）. Under high power microscope field of vision （200x, 0.739 mm^2）, the mean of MVD was 35.8＋13.6 （13-64）. ^11C-eholine SUVmax, did not correlate with tumor size and MVD. ^18F-FDG SUVmax significantly and positively related to MVD （r= 0.525, P=0.002）. There was a eritieal positive correlation between ^18F-FDG SUVmax and tumor size （r=0.335, P=0.057）. Conclusions In the rabbit VX2 lung tumor model, ^18F-FDG SUVmax correlated with MVD, so ^18F- FDG PET-CT could reflect tumor angiogenesis. ^11C-eholine SUVmax did not statistically correlate with MVD, and ^11C-choline PET-CT could not reflect tumor angiogenesis.

关 键 词：肺肿瘤 正电子发射断层显像 氟脱氧葡萄糖F18 ^11C-胆碱 模型 动物 兔 微血管密度 

分 类 号：R734[医药卫生—肿瘤]