紫外可见分光光度法测定共轭亚油酸含量  

Determination of Content of Conjugated Linoleic Acid by UV-Vis Spectrophotometry

作　　者：李泳[1] 张兆霞[1] 

机构地区：[1]广东海洋大学理学院,广东湛江524088

出　　处：《化学与生物工程》2010年第11期92-94,共3页Chemistry & Bioengineering

基　　金：广东省科技基金资助项目(2008B030301269);广东海洋大学博士启动基金资助项目(0512101)

摘　　要：用紫外可见分光光度法测定了由海蓬子油合成的共轭亚油酸含量。利用紫外可见分光光度计进行标准测定,建立了标准回归方程。共轭亚油酸乙酯的最大吸收波长为234 nm,共轭亚油酸乙酯吸光度(A)与共轭亚油酸乙酯浓度(c)间的标准回归方程为A=0.0617+53.3162c(R2=0.9766),结合回归系数获得经验公式:共轭亚油酸浓度c(mol.L-1)=0.0188A。并利用该方法测定了几种合成试样的共轭亚油酸含量,结果表明用紫外可见分光光度法可以快速、准确地测定海蓬子油中共轭亚油酸的含量。The content of conjugated linoleic acid（CLA） in salicorne oil was determined by UV-Vis spectrophotometry.The maximum absorbance wavelength of conjugated linoleic acid ethyl ester was 234 nm.The standard regression equation of absorbance（A） and concentration（c） of conjugated linoleic acid ethyl ester was A=0.0617＋53.3162 c（R2=0.9766）.Based on regression coefficient,the empirical formula c（mol·L-1）=0.0188A for CLA was obtained.The CLA contents of several samples were determined and the results showed that the UV-Vis spectrophotometry could be applied to determine the CLA content in salicorne oil rapidly and accurately.

关 键 词：紫外可见分光光度法 标准回归方程 共轭亚油酸 

分 类 号：O657.32[理学—分析化学]