普城沙雷氏菌Z10发酵工艺参数的研究  

Studies on fermentation technical parameters of Serratia plymuthica Z10

作　　者：杨淑君[1] 陈秀蓉[1] 

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《甘肃农业大学学报》2010年第5期100-103,109,共5页Journal of Gansu Agricultural University

基　　金：甘肃省科技支撑项目(090NKCA082)

摘　　要：通过对普城沙雷氏菌Z10(Serratia plymuthica)在发酵罐中的发酵温度、pH、通气量的研究,得出沙雷氏菌Z10发酵工艺的优化培养条件为:温度28℃、pH7.5、通气量1000L·h-1,在12h补加10%的培养基.在上述优化发酵条件下培养4h,沙雷氏菌Z10进入对数生长期,16h时达到稳定期,最终浓度可达10.632×1012个·mL-1.The effects of fermentation temperature,pH and ventilation size on the fermentation of Serratia plymuthica Z10 were studied.The results indicated that the optimum incubated temperature,pH and ventilation size were 28℃,7.5 and 1 000L·h^-1,respectively,and added 10% culture media after 12h fermented.The growth curve was made by fermentation tank test in the optimum conditions,and it showed that after 4hand 16hculture,the bacteria arrived logarithmic phase and stabilization phase respectively,and the maximal concentrations of live bacteria was 10.632×10^12 individuals·mL^-1 in the fermentation liquid.

关 键 词：沙雷氏菌 发酵 工艺参数 生长曲线 

分 类 号：S476[农业科学—农业昆虫与害虫防治]