血管内皮生长因子-C蛋白和mRNA在胆管癌中的表达及其意义  

The expression and significance of vascular endothelial growth factor-C in cholangioncarcinoma

作　　者：刘三光[1] 穆宏凌[3] 边伟[1] 张树彬[1] 高翔[2] 刘润田[1] 刘建华[1] 

机构地区：[1]河北医科大学第二医院肝胆外科,石家庄050000 [2]河北医科大学第二医院血管外科,石家庄050000 [3]河北省卫生厅考试培训中心

出　　处：《中华实验外科杂志》2010年第11期1657-1659,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 检测胆管癌和癌旁0.5 cm胆管组织及手术切缘的正常胆管组织血管内皮生长因子（VEGF）-C蛋白及mRNA的表达,探讨其在胆管癌发生发展中的作用.方法 应用免疫组织化学过氧化物酶标记链霉卵白素法（SP）检测42例胆管癌组织及20例正常组织中VEGF-C蛋白的表达,同时应用逆转录-聚合酶链反应（RT-PCR）技术检测42例术中所取的新鲜的胆管癌、17例同个体癌旁胆管黏膜和20例正常胆管组织中VEGF-C的mRNA表达,并与临床资料进行相关分析.结果 正常胆管组织、癌旁组织、胆管癌组织中VEGF-C mRNA相对表达量分别0.6105±0.0577、0.6270 ±0.0664、0.6930±0.1078,VEGF-C mRNA在3组之间表达呈上升趋势（P＜0.05）.VEGF-C蛋白表达趋势同其相对应的基因表达趋势一致,即VEGF-C mRNA在胆管癌组织中高表达（P＜0.05）.胆管癌组织、正常胆管组织中VEGF-C表达的阳性率分别为83.33%和30.00%.结论 VEGF-C基因转录和蛋白可能参与了胆管癌的发生发展过程.Objective To detect the expression of vascular endothelial growth factor （VEGF）-C protein and gene in cholangioncarcinoma and to elucidate its role. Methods The expression of VEGF-C protein was detected by immunohistochemical technique （SP） in 42 cases of cholangioncarcinomas and 20cases of normal bile duct, and that of VEGF-C gene was detected in 42 cases of cholangioncarcinomas, tissues adjacent to cholangioncarcinoma and normal bile duct by using reverse transcription-polymerase chain reaction （RT-PCR）. The correlation was analysed statistically between the expression of VEGF-C protein and mRNA and clinicopathological parameters in cholangioncarcinoma. Results The expression of VEGFC mRNA in normal bile duct, tissues adjacent to cholangioncarcinoma, cholangioncarcinoma was 0. 6105 ±0. 0577, 0. 6270 ± 0. 0664, 0. 6930 ± 0. 1078 respectively, and that of VEGF-C mRNA was higher in cholangioncarcinomas than the others （P 〈 0. 05 ）. The protein expression of VEGF-C was higher in cholangioncarcinoma than normal bile duct tissue （P 〈 0. 05 ）. Conclusion VEGF-C gene transcription and protein expression are mostly like involved in the proliferation and malignant transformation of bile epithelial cells and then malignant progression of bile duct carcinomas. The detection of VEGF-C expression may elucidate the carcinogenesis of bile duct.

关 键 词：胆管癌 VEGF 

分 类 号：R735.8[医药卫生—肿瘤]