复合诱变选育高产氨基酰化酶的米曲霉菌株  

USING COMPOUND MUTAGENESIS METHOD TO PRODUCE ASPERGILLUS ORYZAE FOR SELECTING HIGH-YIELD AMINOACYLASE

作　　者：刘丽萍[1] 刘丽华[1] 李文彬[1] 王霞[1] 

机构地区：[1]内蒙古工业大学化工学院生物工程系,呼和浩特市010051

出　　处：《内蒙古工业大学学报（自然科学版）》2010年第3期206-210,共5页Journal of Inner Mongolia University of Technology：Natural Science Edition

基　　金：内蒙古自然科学基金资助项目(200711020206)

摘　　要：选用稀土硝酸镧和紫外线对米曲霉CICC-2339进行联合诱变,通过三角瓶发酵法筛选得到氨基酰化酶高产菌株SUVLa,其酶活提高率为35.13%。经遗传稳定性试验验证,遗传特性稳定。First,aspergillus oryzae（2339-1） is produced by way of compound mutagenesis through the use of rare earth and UV light.Then,a fermentation screening method is adopted and a high-yield aminoacylase strain SUVLa is selected.In comparison with the parent strain,the enzyme activity is enhanced by 35.13%.Hereditary characters of the cultured strain are proved stable by the genetic stability experiment.

关 键 词：米曲霉 复合诱变 氨基酰化酶 

分 类 号：TU472.5[建筑科学—结构工程]