EB病毒潜伏膜蛋白LMP-1在鼻咽癌中的表达及临床意义  

Expressions of LMP-1 in Nasopharyngeal Carcinoma and its Clinical Significance

作　　者：林锦[1] 郭巧娟[1] 韩露[1] 陈奇松[1] 宗井凤[1] 潘建基[1] 林少俊[1] 

机构地区：[1]福建医科大学教学医院,福建省肿瘤医院放疗科,350014

出　　处：《实用癌症杂志》2010年第6期564-567,共4页The Practical Journal of Cancer

基　　金：福建省卫生厅医学创新课题基金(2007-CXB-4)

摘　　要：目的观察EB病毒(EBV)潜伏膜蛋白1(LMP-1)在鼻咽癌(NPC)鼻咽拭子中的表达,探讨LMP-1表达与NPC临床病理特征的关系。方法纳入2007年～2008年福建省肿瘤医院放疗科收治的经病理检查确诊为NPC且同意参加该试验的初治患者129例,均在治疗前应用鼻咽拭子法取鼻咽部黏膜脱落细胞,提取DNA,采用PCR法检测LMP-1基因的表达。结果 129例患者中有114例患者LMP-1呈阳性表达,另15例呈阴性表达,阳性率为88.37%(114/129);对照组中无1例呈阳性表达。LMP-1阳性表达率有颈部淋巴结转移组明显高于无颈部淋巴结转移组。LMP-1表达与患者年龄、性别、临床分期、T分期及M分期无相关性。结论 PCR法检测鼻咽拭子中LMP-1的检出率高于免疫组化法,LMP-1表达与鼻咽癌患者颈部淋巴结转移密切相关,临床中可预测鼻咽癌患者的转移潜能,LMP-1可能做为抗肿瘤转移靶向治疗的潜在靶点。Objective To investigate the clinical significance of Epstein-Barr virus（EBV） latent membrane protein-1（LMP-1） in nasopharyngeal carcinoma（NPC）.Methods 129 patients with pathologically confirmed NPC between 2007 and 2008 were involved in this study.After informed consent,nasopharyngeal swab was performed to obtain cells for the LMP-1 PCR assay.Results Of the 129 patients,LMP-1 was expressed in 114（88.37%）.The positive expression rate of LMP-1 in patients with neck lymph node metastasis was significantly higher than that without neck lymph node metastasis（P 0.05）.The expression of LMP-1 was not correlated with age,gender,clinical stage,T stage,M stage and histological type（P 0.05）.Conclusion The detection rate of positive LMP-1 expression was higher by PCR assay than that by immunohistochemisty.The positive expression of LMP-1 was highly correlated with lymph node metastasis.It could be used to predict the metastatic potential of NPC,and further be used as a potential target for anti-metastasis therapy.

关 键 词：鼻咽癌 EB病毒 潜伏膜蛋白LMP-1 

分 类 号：R739.63[医药卫生—肿瘤]