广东仁化白毛茶优异株系遗传多样性的EST-SSR分析  

Genetic diversity of EST-SSR in Renhua baimaocha in Guangdong

作　　者：乔小燕[1] 吴华玲[1] 陈栋[1] 李家贤[1] 黄华林[1] 晏嫦妤[1] 何玉媚[1] 

机构地区：[1]广东省农科院茶叶研究所,广东广州510640

出　　处：《广东农业科学》2010年第11期57-59,共3页Guangdong Agricultural Sciences

基　　金：国家农业(茶叶)产业技术体系建设专项;广东省农业厅农业科技计划项目(200844502);广东省农业厅农业推广专项(粤农函[2009]1124号);广东省科技计划项目(2009B020303003)

摘　　要：以13份白毛茶茶树资源为供试材料,应用32对EST-SSR引物进行遗传多样性研究。结果表明,供试茶树品种均表现出多态性,共检测到71个等位位点,平均每对引物可检测到2.3个。等位位点的观测杂合度平均值为0.30,期望杂合度平均值为0.34,引物扩增位点的多态性信息量平均为0.39,基因多样性指数为0.43。从UPGMA聚类图中可以看出,13份白毛茶单株系可分为3个类群,大叶青、大叶黄独自聚为第Ⅰ类群;丹霞4号、特白、丹霞7号、丹霞2号、丹霞11号、丹霞13号和丹霞14号聚为第Ⅱ大类群,丹霞1号、丹霞5号、丹霞6号和丹霞8号聚为第Ⅲ类群。Renhua Baimaocha 〔Camellia sinensis（L.） O.Kuntze〕 is the main cultivars in Guangdong.Thirteen lines as the experimental materials,32 core primers were selected and used for genetic diversity.All selected primers were polymorphic and 71 alleles were amplified with 2.3 alleles per primer pair on an average.The mean of polymorphism information content（PIC） was 0.39,with the mean genetic diversity of 0.43.Observed heterozygosity（Ho） was 0.30,while expected heterozygosity（He） was 0.34.UPGMA cluster analysis showed that 13 cultivars were divided into three groups.Dayeqing and Dayehuang were clustered into groupⅠ.Danxia 4,Tebai,Danxia 7,Danxia 2,Danxia11,Danxia13 and Danxia14 cultivars were clustered into group Ⅱ according to their pedigree.Danxia 1,Danxia 5,Danxia 6 and Danxia 8 went into group Ⅲ.

关 键 词：茶树 白毛茶 遗传多样性 EST-SSR 

分 类 号：S661.2[农业科学—果树学]