ICL、ICDH、ODH和GDH酶与L-谷氨酸的合成  

Effect of ICL,ICDH,ODH and GDH on L-Glutamate Production

作　　者：余秉琦[1] 朱劼[1] 何玉财[1] 蔡志强[1] 王利群[1] 杨林松[1] 

机构地区：[1]常州大学化学化工学院,江苏常州213164

出　　处：《安徽农业科学》2010年第30期16725-16727,16730,共4页Journal of Anhui Agricultural Sciences

摘　　要：通过对对数生长期细胞的异柠檬酸裂解酶(ICL)、异柠檬酸脱氢酶(ICDH)、α-酮戊二酸脱氢酶(ODH)和谷氨酸脱氢酶(GDH)的分析以及对细胞L-谷氨酸产生量和发酵残糖的综合分析,发现由于乙醛酸循环关键酶ICL的缺失,L-谷氨酸产生量大幅下降,说明乙醛酸循环是谷氨酸棒杆菌中的主要回补途径,L-谷氨酸合成需要乙醛酸循环。在保证乙醛酸循环回补功能的前提下,提高ICDH活性,减弱ODH活性,有利于L-谷氨酸的合成。Investigation into relationship between L-glutamate production and enzymes isocitrate lyase（ICL）,isocitrate dehydrogenase（ICDH）,2-oxoglutarate dehydrogenase（ODH）as well as glutamate dehydrogenase（GDH） from cells in the logarithmic growth phase was carried out.The results indicated that the glyoxylic cycle was the main anaplerotic pathway and indispensable for L-glutamate production in C.glutamicum.Furthermore,it was revealed that L-glutamate production could be stimulated when ICDH activity increased and ODH activity decreased in existence of the glyoxylic anaplerotic pathway.

关 键 词：谷氨酸 异柠檬酸裂解酶 异柠檬酸脱氢酶 α-酮戊二酸脱氢酶 谷氨酸脱氢酶 

分 类 号：S188[农业科学—农业基础科学]