南极菌B-3胞外寡聚糖对黄瓜的诱导抗病作用  

Induced Resistance against Cucumber by Antarctic Bacterium Psychrobacter sp.B-3 Oligosaccharides

作　　者：谭姣姣[1,2] 李江[2] 何培青[2] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042 [2]国家海洋局第一海洋研究所海洋生物活性物质重点实验室,山东青岛266061

出　　处：《安徽农业科学》2010年第30期16903-16905,共3页Journal of Anhui Agricultural Sciences

基　　金：国家"863"科技计划重点项目"极地微生物资源利用关键技术研究"(2007AA091905)

摘　　要：[目的]研究南极菌B-3对黄瓜的诱导抗病作用。[方法]以黄瓜幼苗为材料,研究南极菌B-3胞外寡聚糖的分子量、单糖组成,及对黄瓜叶中防御酶的诱导作用与黄瓜白粉病的诱导抗病性。[结果]B-3胞外寡聚糖经高效凝胶渗透色谱测定其分子量为2112D,气相色谱分析由甘露糖和葡萄糖2种单糖组成;0.3%、0.5%和0.8%的B-3胞外多糖分别对黄瓜幼苗喷施不同时间后,黄瓜体内的几丁质酶,β-1,3-葡聚糖酶,苯丙氨酸解氨酶(PAL),过氧化物酶(POD)的活性均较对照有不同程度的提高,且与0.5%的壳聚糖具有相似的诱导效果;同时,0.5%的B-3多糖能够显著地降低黄瓜白粉病的病情指数,防效达24.49%。[结论]B-3寡聚糖有望开发为新型抗病诱导子。[Objective]The aim was to study the induction and resistant effect of antarctic bacterium Psychrobacter sp.B-3 oligosaccharides against cucumber.[Method]The molecular weight,sugar composition,induced resistance against powdery mildew and defense enzymes activities related to resistance in cucumber seedlings treated with self-developed Antarctic bacteria B-3 extracellular oligasaccharides were studied.[Result]The molecular weight of B-3 extracellular oligasaccharides was determined as 2 112 D by the HPLC method,sugar composition revealed that main structure of B-3 was mannose and glucose;the activities of chinitase,β-1,3-glucanse,phenylanine,ammonialyse（PAL）,superoxide dismutase（SOD）,and peroxidase（POD） in cucumber seedlings stimulated by B-3 increased apparently at different times compared with untreated plants,and B-3 has a similar induction effects with 0.5% of the chitosan; at the same time,0.5% of the B-3 polysaccharide significantly reduced the disease index of cucumber powdery mildew,anti-efficiency reached 24.49%.[Conclusion]B-3 polysaccharide is expected to develop as a new type of resistance elicitor.

关 键 词：PSYCHROBACTER sp.B-3 寡聚糖 组成分析 诱导 防御酶 

分 类 号：S436.421[农业科学—农业昆虫与害虫防治]