Bmi1基因在小鼠睾丸组织中的表达  

Expression of Bmi1 in mouse testis

作　　者：张世庆[1,2] 李澜[1] 鲁景艳[1] 李德雪[1] 

机构地区：[1]军事医学科学院,北京100850 [2]国家食品药品监督管理局医疗器械技术审评中心,北京100044

出　　处：《军事医学科学院院刊》2010年第5期419-422,446,共5页Bulletin of the Academy of Military Medical Sciences

基　　金：国家自然科学基金(30972090);总后卫生部指令性项目(08ZL030)

摘　　要：目的分析Bmi1基因在小鼠睾丸组织中的表达。方法利用免疫组织化学和免疫荧光共定位及流式细胞术对Bmi1在小鼠睾丸中的表达位置进行检测;利用实时荧光定量PCR对不同日龄小鼠睾丸中Bmi1的表达变化进行分析。结果实验数据显示,Bmi1基因表达于精原干细胞;从第2天开始,Bmi1的表达急剧增加,第8天开始急剧下降,第24天时趋于平稳。结论Bmi1基因可能是精原干细胞增殖的关键调控因子。Objective To analyze the expression of Bmi1 in mouse testis.Methods Flow cytometric analysis and immunofluorescence localization were used to localize the exact expression position while real-time immunofluorescence quantity PCR was used to analyze the expression variety of Bmi1 in mouse testis.Results Data showed that Bmi1 was expressed in spermatogonial stem cells.The expression quantity was accelerated from the 2nd day and cut down rapidly from 8th day.The expression tended to remain steady from 24th day.Conclusion Bmi1 gene might be the key self-proliferation regulator of spermatogonial stem cells.

关 键 词：精原细胞 BMI1 自增殖 睾丸 

分 类 号：R363[医药卫生—病理学]