土大黄苷对乳腺癌细胞SK-BR-3增殖的影响  

Effects of rhaponticin on proliferation of SK-BR-3 breast cancer cells

作　　者：赵素容[1] 段海峰[2] 许尹[2] 卢兖伟[2] 蒋志文[1] 

机构地区：[1]蚌埠医学院药学系,安徽蚌埠233030 [2]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《军事医学科学院院刊》2010年第5期444-446,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：安徽省高校省级优秀青年人才基金(2010SQRL122);蚌埠医学院科研项目(BY0823)

摘　　要：目的观察土大黄苷对乳腺癌细胞SK-BR-3增殖的影响,并初步探讨其作用机制。方法采用MTT法检测土大黄苷对SK-BR-3细胞的增殖抑制作用,倒置显微镜下观察细胞的形态学变化,W estern印迹检测Akt及p-Akt蛋白的表达情况。结果土大黄苷可抑制SK-BR-3细胞增殖,其作用具有浓度和时间依赖性,72 h的IC50为(181.3±11.4)μmol/L。200、400μmol/L土大黄苷作用48 h后显示出明显的细胞毒作用,显微镜下可见细胞碎片增多、细胞密度减小。土大黄苷在100、200、400μmol/L浓度时均可抑制p-Akt蛋白的表达。结论土大黄苷可抑制SK-BR-3细胞的增殖,其机制可能与抑制p-Akt表达有关。Objective To investigate the effect of rhaponticin on the proliferation of SK-BR-3 cells,and to probe the related mechanism.Methods The effect of rhaponticin on proliferation of SK-BR-3 cells was assessed by MTT assay,the morphologic changes were observed by light microscopy,and the expression of Akt and p-Akt protein was analyzed by Western blot.Results Rhaponticin inhibited the proliferation of SK-BR-3 cells in a dose-and time-dependent manner,with an IC50 of（181.3±11.4） μmol/L at 72 h.Obvious cytotoxicity was observed in SK-BR-3 cells after exposure to rhaponticin at the concentration of 200 and 400 μmol/L for 48 h.Rhaponticin could also inhibit the expression of p-Akt protein at the concentration of 100,200 and 400 μmol/L.Conclusion Rhaponticin can inhibit the proliferation of SK-BR-3 cells,which may be correlated with the down-regulation of p-Akt protein.

关 键 词：土大黄苷 乳腺肿瘤 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]