肝癌中Sall3基因甲基化的研究  

Methylation of Sall3 gene in hepatocellular carcinoma

作　　者：夏卫 倪唯益 费琦[2] 孙晋枫[2] 赵仰星[2] 张宏宇[2] 顾峻[2] 何英华[2] 余坚[2] 

机构地区：[1]上海市静安区石门二路街道社区卫生服务中心检验科,上海200041 [2]上海交通大学肿瘤研究所癌基因及相关基因国家重点实验室肿瘤表观遗传学组,上海200032

出　　处：《诊断学理论与实践》2010年第5期491-494,共4页Journal of Diagnostics Concepts & Practice

摘　　要：目的:检测Sall3基因在肝癌细胞系和肝癌组织中的甲基化状况及其表达水平。方法:对7株肝癌细胞系、142对肝癌组织和6例正常肝组织进行Sall3基因启动子区域甲基化检测。甲基化研究使用甲基化特异性聚合酶链反应(methylation specific polymerase chain reaction,MSP),基因表达水平研究采用实时定量反转录PCR(real-time RT-PCR)。结果:MSP结果显示,7株肝癌细胞株中有6株Sall3基因的启动子区域为纯合甲基化,1株为纯合去甲基化;在6例正常肝组织中Sall3均为纯合去甲基化;在142对肝癌组织标本中,癌旁组织Sall3基因启动子区域甲基化率为18.31%,在癌组织中为64.79%。real-time RT-PCR结果显示,肝癌肿瘤细胞系Sall3mRNA的表达水平明显低于去甲基化的正常肝组织。结论:Sall3基因启动子甲基化在正常肝组织、肝癌组织(和肝癌细胞系)中有明显差异。Objective To detect the methylation status and expression of Sall3 gene in hepatocellular carcinoma cell lines and cancer tissues. Methods Methylation-specific polymerase chain reaction （MSP） was used to define the methylation pattern of Sall3 and real-time RT-PCR was used to detect the expression of Sall3 in seven hepatocellular carcinoma cell lines, paired cancer and non-cancerous tissues of 142 HCC patients and 6 normal liver specimens. Results MSP showed that 6 of 7 hepatocellular carcinoma cell lines had homozygous methylation of Sall3 gene promotor region and had homozygous demethylation in one cell line. By contrast, demethylation was detected in all 6 normal liver specimens. Of the 142 paired cancer and non-cancerous tissues, the methylation rate in cancer tissue was 64.79% （92/142） and in non- cancerous tissue was 18.31% （26/142）. Furthermore, real-time RT-PCR demonstrated that the expression level of Sall3 mRNA in high methylation liver carcinoma cell lines was significantly lower that in normal liver tissue. Conclusions Significant difference is seen in methylation of Sall3 between hepatocellular carcinoma （both cancer tissues and cell lines） and normal liver tissues.

关 键 词：肝癌 Sall3 甲基化 甲基化特异性聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]