卵母细胞mtDNA4977bp缺失在卵巢储备功能下降中的研究  

Increased mitochondrial DNA 4977 bp deletion in unfertilized oocytes of patients with diminished ovarian reserve

作　　者：赵红翠[1,2] 李艳萍[1] 

机构地区：[1]中南大学湘雅医院妇产科生殖医学中心,长沙410008 [2]解放军306医院生殖医学中心,北京100101

出　　处：《生殖医学杂志》2010年第5期406-410,共5页Journal of Reproductive Medicine

基　　金：湖南省科厅科技计划一般项目(05FJ4150)

摘　　要：目的检测卵巢储备功能下降患者卵母细胞线粒体DNA(mtDNA)4977 bp缺失,探讨其对卵巢储备功能下降患者生育潜能的可能影响。方法选取湘雅医院生殖中心2008年3～9月行体外受精-胚胎移植(IVF-ET)治疗的不育患者80例,按卵巢储备功能状态分为卵巢储备功能下降(DOR)和卵巢储备功能正常(NOR)两组,胚胎移植日收集两组患者成熟未受精MII卵母细胞110枚,DOR组46枚,NOR组64枚。采用聚合酶链反应(PCR)定性检测mtDNA 4977 bp缺失,比较两组患者卵母细胞mtDNA 4977 bp缺失率。结果 DOR组与NOR组比较,卵母细胞具有较高的mtDNA 4977 bp缺失率(48.8%vs 18.8%,P<0.05),两者比较差异有显著性。Logistic回归分析显示,患者年龄对两组患者mtDNA4977 bp的检测结果无影响。结论 DOR患者卵母细胞mtDNA4977 bp缺失率增高,可能与其卵母细胞发育潜能下降相关。Objective： To detect mitochondrial DNA （mtDNA） 4977 bp deletion in the oocytes of the patients with diminished ovarian reserve, and help to understand its impaction on the reproductive potential. Methods： Eighty infertile patients treated with in vitro fertilization-embryo transfer （IVF-ET） from Mar to Sept. 2008 in Xiangya Hospital were recruited and divided into two groups according to ovarian reserve： diminished ovarian reserve （DOR, with basal follicular stimulating hormone （bFSH） levels 12 U/L and/or bilateral antral follicle count （AFC） 45） and normal ovarian reserve （NOR, with bFSH levels〈8 U/L, luteinizing hormone （LH） levels 〈6 U/L, estradiol levels of 110-184 pmol/L, and unilat- eral AFC 3-10）. 110 unfertilized MII oocytes were collected on the day of embryo transfer （46 in DOR group, 64 in NOR group） and mtDNA 4977 bp deletion was detected by polymerase chain reaction （PCR）. Results： A significantly higher rate of mtDNA 4977 bp deletion was observed in the MII oocytes in DOR group （48.8%） than in NOR group （18.8%）（P〈0.05）. Logistic regression analysis showed that patients＇ age was not significantly correlated with the deletion rate. Conclusions： The higher mtDNA4977bp deletion rate in DOR patients＇ ooeytes may be associated with their decreased oocyte developmental potential.

关 键 词：卵母细胞 卵巢储备功能 MTDNA 4977 bp缺失 

分 类 号：R[医药卫生]