1株亚硝酸盐降解菌的筛选、鉴定、降解条件及效果  

Screening,identification,degradation condition and effects of a nitrite-degradating strain

作　　者：熊焰[1] 刘力源[1] 罗睿[1] 朱欢[1] 

机构地区：[1]四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国水产科学》2010年第6期1264-1271,共8页Journal of Fishery Sciences of China

基　　金：教育部<长江学者和创新团队发展计划>创新团队基金资助项目(IRTO848)

摘　　要：从养殖水体中筛选出1株对亚硝酸盐具有高效降解能力、增殖速度快、稳定和安全的优良菌株,经细菌学常规检测和16SrRNA序列分析确定为巨大芽孢杆菌(Bacillus megaterium),并命名为SZ-3。对SZ-3菌株降解条件的单因素实验发现,其最佳降解条件是:pH7.0,温度33℃,降解所需的最低葡萄糖含量为0.01mg/L,在24h内对亚硝酸盐的降解率达69.58%。在亚硝酸盐质量浓度为5mg/L的100L污染水体养殖实验中,添加1000mL含量为1×108CFU/mL的SZ-3培养菌液离心后的纯菌体,在28℃的水温条件下经96h,SZ-3菌株对亚硝酸盐的降解率可达91.78%。表明该菌株对污染水体中的亚硝酸盐具有较强的降解效果。本研究旨在为研发高效实用的降解亚硝酸盐微生态活性菌奠定基础。An efficient,rapid multiplicative,stable and safe nitrite-degradating strain was selected from aquatic water.According to the conventional physiological,biochemical measurement and the 16S rRNA sequence analysis,the strain was identified as Bacillus megaterium and named SZ-3.Based on the single-factor optimization tests to the strain,the optimum degradation conditions are found as follows,the optimal pH value is 7,the perfect temperature is 33 ℃ and the minimum glucose require ment of degradation is 0.01 mg/L.Under these circumstances,the degradation rate of SZ-3 strain to nitrite came up to 69.58% in 24 h.In breeding test of 100 L polluted water in which the concentration of nitrite is 5 mg/L,the degradation rate of SZ-3 strain on the nitrite achieved at 91.78% for 96 h in 28 ℃ via adding the pure thalli of SZ-3 which was obtained by centrifugated 1 000 mL（1×108 CFU/mLl） culture fluid.All of these tests above indicate that the strain can degrade the nitrite in polluted water efficiently.

关 键 词：菌株筛选 巨大芽孢杆菌 亚硝酸盐 污染水体 

分 类 号：S94[农业科学—水产养殖]