Rho-GDIα抑制尼古丁诱导的血管平滑肌细胞迁移  

Rho-GDIαInhibited the Migration of Vascular Smooth Muscle Cells Induced by Nicotine in Rats

作　　者：曲明娟[1] 舒霏霏[1] 靖美东[1] 张莉[1] 

机构地区：[1]鲁东大学生命科学学院,烟台264010

出　　处：《中国细胞生物学学报》2010年第5期733-737,共5页Chinese Journal of Cell Biology

基　　金：鲁东大学人才引进基金(No.LY20083302);鲁东大学学科建设经费资助项目~~

摘　　要：研究尼古丁对大鼠胸主动脉血管平滑肌细胞(vascular smooth muscle cells,VSMCs)迁移的影响和机制,为探讨吸烟加速动脉粥样硬化等心血管疾病的机制研究提供实验依据。用终浓度为100μmol/L的尼古丁作用于体外培养的大鼠VSMCs 24 h,以不加尼古丁的细胞为对照组,采用迁移小室法(Transwell)和划痕法检测有或无尼古丁条件下VSMCs的迁移情况,实时定量RT-PCR检测Rho家族活性调节因子Rho-GDIα的表达;RNA干扰抑制Rho-GDIα的表达,探讨其在尼古丁诱导的VSMCs迁移中的作用。迁移小室法和划痕法分别显示了尼古丁组的VSMCs迁移数量和距离明显比对照组的多和远;尼古丁显著抑制Rho-GDIα的mRNA表达;RNA干扰Rho-GDIα后,VSMCs的迁移能力明显提高。结果提示,尼古丁可能部分通过抑制Rho-GDIα的表达促进VSMCs的迁移。To investigate the mechanism of the smoking-induced progress and mechanism of the atherosclerosis,the effect of nicotine on the migration of vascular smooth muscle cells（VSMCs） of rats was studied.Vascular smooth muscle cells（VSMCs） isolated from rats were subjected to 100μmol/L nicotine for 24 hours,and cells treated without nicotine were used as control.The migration ability of VSMCs was checked by Transwell assay and Wound healing assay,respectively.The mRNA expression of Rho-GDIαwas detected using RNA interference（RNAi） and Real-time RT-PCR.The migration ability of VSMCs was significantly promoted by nicotine stimulation compared with the control group.The mRNA expression of Rho-GDIαwas depressed by nicotine treated as well as RNAi.It is concluded that nicotine can promote the migration of VSMC in vitro partly through inhibiting the expression of Rho-GDIα,which explicits the role of nicotine in the atherosclerosis.

关 键 词：尼古丁 血管平滑肌细胞 迁移 鸟苷酸分离抑制因子 RNA干扰 

分 类 号：R363[医药卫生—病理学]