基质细胞衍生因子在哮喘小鼠气道中的表达  

Expression of stromal-derived factor-1 in the airway of mice with allergic asthma

作　　者：黄芸[1,2] 欧阳海峰[1] 彭晓丹[2] 王文雅[1] 吴昌归[1] 

机构地区：[1]第四军医大学西京医院呼吸内科,陕西西安710032 [2]中国人民解放军第533医院,云南昆明650225

出　　处：《西安交通大学学报（医学版）》2010年第6期690-692,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.30770927)~~

摘　　要：目的观察基质细胞衍生因子-1(stromal-derivedfactor-1,SDF-1)在鸡卵清蛋白(OVA)诱导小鼠哮喘模型中的表达,探索哮喘气道重塑的发生机制。方法 40只雌性SPF级C57BL/6小鼠,体重18～22 g,随机分为哮喘组和对照组。按照文献方法建立OVA小鼠哮喘模型。两组动物于末次雾化结束后24 h处死,取肺组织行病理切片,苏木精-伊红染色(HE)观察肺脏的组织病理学变化;逆转录聚合酶链式反应(RT-PCR)、免疫印迹法(Western blot)、免疫组织化学(immunohistochemistry,I H)技术检测肺组织SDF-1的表达。结果①哮喘组支气管上皮细胞脱落、气道壁增厚,细支气管和血管周围有大量炎细胞浸润;②哮喘组肺组织SDF-1在RT-PCR、Western blot及I H中均为阳性表达,而对照组则否。结论小鼠哮喘时肺组织分泌大量细胞趋化因子SDF-1,可能是哮喘气道重塑的重要因素之一。Objective To study the expression of stromal-derived factor-1（SDF-1） in the airway of mice with allergic asthma induced by ovalbumin（OVA） so as to explore the mechanism of asthmatic airway remodeling.Methods Forty female C57BL/6 mice of SPF grade were randomly divided into asthma group and control group with 20 in each.Mice were sensitized and challenged with ovalbumin to establish the asthmatic model.All the mice were killed 24 hours after the last aerosolisation.The paraffin embedded sections of lung tissues were stained with hematoxylin and eosin（HE） for analyzing pathological changes.SDF-1 mRNA extracted from lung tissues was assessed by RT-PCR while SDF-1 protein extracted from lung tissues was assessed by Western blot and immunohistochemistry（IH）.Results ① After repeated allergen challenge,obvious infiltration of inflammatory cells and proliferation of goblet cells and smooth muscles appeared in mouse bronchi.② According to RT-PCR and Western blot and IH results,the expression of SDF-1 was positive in asthma group but negative in control group.Conclusion A large amount of SDF-1 is secreted in the lung tissues in asthmatic mice,which may be one of the critical factors in asthmatic airway remodeling.

关 键 词：基质细胞衍生因子 哮喘 气道重塑 

分 类 号：R363[医药卫生—病理学]