奥沙利铂通过上调死亡受体表达增强TRAIL诱导胃癌细胞凋亡的实验研究  

Oxaliplatin enhances TRAIL-induced apoptosis in gastric cancer cells by upregulating the expression of death receptors

作　　者：徐玲[1] 刘静[1] 刘云鹏[1] 曲秀娟[1] 张晔[1] 侯科佐[1] 姜又红[2] 

机构地区：[1]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001 [2]中国医科大学附属第一医院肿瘤研究所第二研究室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2010年第11期2085-2088,共4页Journal of Modern Oncology

基　　金：国家自然科学基金资助项目(No.30770993)

摘　　要：目的:研究奥沙利铂对TRAIL诱导胃癌细胞凋亡的影响,探讨死亡受体5(DR5)在TRAIL诱导细胞凋亡中的作用。方法:采用MTT法测定细胞活力、采用流式细胞仪检测细胞凋亡,采用Western blot检测DR5蛋白表达。结果:100ng/ml的TRAIL导致轻度的增殖抑制,诱导不超过5%的细胞凋亡;TRAIL(100ng/ml)联合奥沙利铂(23.44μg/ml)引起明显的细胞增殖抑制和细胞凋亡(P<0.05),TRAIL没有明显改变死亡受体5(DR5)的表达,而23.44μg/ml奥沙利铂作用胃癌细胞24小时后,明显上调了DR5的表达。结论:奥沙利铂通过上调DR5的表达增强TRAIL诱导的胃癌细胞凋亡。Objective：To investigate the effect of Oxaliplatin on TRAIL-induced apoptosis in human gastric cancer cells,and the role of death receptor 5（DR5） in TRAIL-induced cell apoptosis.Methods：Cell proliferation was measured using MTT assay.Cell apoptosis was determined by flow cytometry.Expression of proteins was analyzed by Western blot.Results：100 ng/ml TRAIL resulted in a slight reduction in cell viability in MGC803 cells.Treatment with TRAIL alone caused no more than 5% cell apoptosis.Treatment with TRAIL（100 ng/ml） and Oxaliplatin（23.44 μg/ml） for 24 h resulted in a significant reduction in cell viability and a dramatic increase in cell apoptosis（P0.05）.TRAIL alone did not significantly change the expression of death receptor 5（DR5）,while 23.44μg/ml Oxaliplatin significantly upregulated DR5 expression at 24 h.Conclusion：Oxaliplatin enhanced TRAIL-induced gastric cancer cell apoptosis through upregulating DR5 expression.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 奥沙利铂 胃癌 凋亡 死亡受体 

分 类 号：R730.2[医药卫生—肿瘤] R735.2[医药卫生—临床医学]