单个核细胞与H460细胞相互作用对MMP-1,MMP-3表达的影响  

Influence of interactions between mononuclear cells and lung cancer cells on the expression of MMP-1,MMP-3

作　　者：闫江涛[1,2] 朱运奎[1] 王晓芹[1] 刘卫[1] 李继东[1] 

机构地区：[1]兰州军区兰州总医院呼吸内科,甘肃兰州730050 [2]兰州大学第二临床医学院,甘肃兰州730050

出　　处：《现代肿瘤医学》2010年第11期2111-2114,共4页Journal of Modern Oncology

基　　金：甘肃省自然科学研究基金资助项目(编号:SZS051-A25-088)

摘　　要：目的:研究单个核细胞与肺癌H460细胞在混合培养条件下,相互作用对基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)表达的影响。方法:H460细胞、志愿者血分离出的单个核细胞分别培养于无血清的DMEM中;同时,单个核细胞和H460细胞以1:5的比例混合培养,48小时后,用细胞裂解液(lysisbuffer)裂解细胞,然后收集细胞裂解液采用Western blot法检测MMP-1、MMP-3的表达。结果:在混合培养条件下,单个核细胞与肺癌H460细胞相互作用,能明显促进胞浆内MMP-1、3的表达水平。结论:单个核细胞与肺癌H460细胞相互作用能通过上调MMP-1、3的表达,促进肺癌侵袭和转移。Objective：To explore the influence of interactions between mononuclear cells and lung cancer cells（H460） on matrix metalloproteinase-1,3（MMP-1,3） expression in co-culture condition.Methods： H460 and mononuclear cells-derived from the blood of volunteer were co-cultured in DMEM medium.Mononuclear cells with H460（1：5）were mixed and co-cultured at the same time.After 48 hours,lysissed the cells,the expression of MMP-1,3 in cytoplasm were detected by Western blotting analysis.Result： In co-culture,mononuclear cells interacting with H460 cells can increased the secretions of MMP-1 and 3 in cytoplasm.Conclusion：The interactions between mononuclear cells and lung cancer cells may promote the invasion and metastasis of lung cancer through up-regulating the secretion and activation of MMP-1,MMP-3.

关 键 词：H460细胞 单个核细胞 MMP-1、3 侵袭 转移 

分 类 号：R73-351[医药卫生—肿瘤] R734.2[医药卫生—临床医学]