基因芯片在早期预测肝癌肝移植转移复发中的应用  

Differential gene expression related to liver cancer recurrence after liver transplantation:30 cases of CDNA microarray analysis

作　　者：郭志刚 余元龙[1] 胡泽民[1] 陈宏[1] 常晓健[1] 熊俊[2] 刘龙山[2] 马毅[2] 

机构地区：[1]中山大学附属中山医院普外三科,广东中山528403 [2]中山大学附属第一医院,广东广州510080

出　　处：《现代肿瘤医学》2010年第11期2198-2200,共3页Journal of Modern Oncology

基　　金：中山市科技局计划项目(编号:B200755)

摘　　要：目的:通过基因芯片技术研究肝癌肝移植后移植肝发生转移的基因表达差异,寻找与转移相关的基因及其分子机制。方法:选取30例因原发性肝癌行肝移植手术患者分为两组,对照组为肝移植手术后两年内未出现移植肝转移者,实验组为手术后两年内出现移植肝转移者。手术后3-24个月内在B超引导下行移植肝穿刺取材检测。结果:两组差异表达的基因有96个,占芯片基因总数的2.34%,表达上调基因57个,表达下调基因39个。其中显著差异表达的基因21个,占差异表达基因的21.88%,上调基因13个,下调基因8个。结论:肝癌肝移植后移植肝发生转移是多种基因联合作用的结果。Objective：To investigate the differential expression of genes related to liver cancer recurrence after liver transplantation.Methods： Thirty patients were enrolled in this study,who received liver transplantation due to primary liver cancer.Liver graft biopsy was performed in all patients at three to 24 month after liver transplant,and cDNA microarray analysis was performed to detect gene expression.According to cancer recurrence of liver graft,30 patients were divided into two groups,cancer recurrence（CR） group and non-cancer recurrence（non-CR） group.Gene expression in two groups was compared and analyzed.Results： Twenty-one graft biopsies were finally used.There were 96 genes of differential expression,which accounted 2.34% of whole set probes.Among these 96 genes,57 were up-regulated and 39 were down-regulated.There were totally 21 genes with significant differentiation,which accounted 21.88%（21/96） of differential expression genes.Among these 21 genes,13 were up-regulated and eight were down-regulated.Conclusion： Liver cancer metastasis and recurrence after liver transplantation is related to multiple genes.

关 键 词：基因芯片 肝癌 肝移植 肝转移 

分 类 号：R735.7[医药卫生—肿瘤]