宫颈脱落细胞hTERC基因表达及其在宫颈病变诊断中的应用  

Clinical application of fluorescence in situ hybridization in cervical lesion

作　　者：刘东涌[1] 魏力[1] 

机构地区：[1]中国医科大学附属第四医院妇科,辽宁沈阳110032

出　　处：《现代肿瘤医学》2010年第11期2239-2241,共3页Journal of Modern Oncology

基　　金：国家卫生部科研资助项目(编号:WKJ2007-3-001);辽宁省科技攻关计划项目(编号:2008225010-11)

摘　　要：目的:初步探讨荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测人类染色体末端酶基因(the human telomerase gene,hTERC)在宫颈病变诊断中的价值及临床意义。方法:应用荧光原位杂交技术检测20例正常人宫颈脱落细胞标本,建立hTERC基因表达的实验阈值;检测100例病理证实为宫颈疾病的患者标本,包括宫颈上皮内瘤样病变I(cervical intraepithelial neoplasia I,CIN1)28例、CIN2 27例、CIN3 17例以及宫颈癌28例。结果:hTERC在正常人组阳性率5.55%;在CIN1、CIN2、CIN3及宫颈癌组表达率分别为21.4%、55.6%、70.6%及92.9%;hTERC基因在CIN1组的表达与CIN2、CIN3及宫颈癌组比较有统计学差异(P<0.05)。结论:hTERC基因的异常扩增与宫颈病变进展程度呈正相关,关联密切,可能成为宫颈病变诊断的早期标志物。Objective：To investigate the expression and clinical value of fluorescence in situ hybridization（FISH） in cervical diseases.Methods： Detecting 20 normal cases by FISH to build the standard threshold;Then detecting 100 patients by FISH including CIN1 28 cases,CIN2 27 cases,CIN3 17 cases and cancer 28 cases.Results： Positive expression rates of hTERC gene in different groups：normal cervixes,CIN1,CIN2,CIN3 and cancers were：5.55%,21.4%,55.6%,70.6% and 92.9%.There was a significant difference between CIN1 and CIN2-Ca（P0.05）.Conclusion： FISH can be used in hTERC gene detection quickly.The abnormal and over expression of hTERC might be a early marker in cervical lesion.

关 键 词：荧光原位杂交 宫颈上皮内瘤样病变 宫颈癌 HTERC基因 

分 类 号：R737.33[医药卫生—肿瘤]