氧化苦参碱注射液联合小剂量紫杉醇对人胃癌SGC-7901细胞血管内皮生长因子及CXC趋化因子受体4表达的影响  

Effects of oxymatrine injection combined with low-dose paclitaxel on mRNA and protein expressions of vascular endothelial growth factor and CXC chemokine receptor 4 in human gastric carcinoma SGC-7901 cells

作　　者：张于人[1] 朱金水[1] 王小银[1] 许志鹏[1] 周洲[1] 王龙[1] 孙群[1] 朱励[1] 

机构地区：[1]上海交通大学第六人民医院消化科,上海200233

出　　处：《中西医结合学报》2010年第11期1029-1035,共7页Journal of Chinese Integrative Medicine

基　　金：基金项目：上海市科学技术委员会科研计划资助项目（No.10140902500）

摘　　要：目的：探讨氧化苦参碱注射液联合小剂量紫杉醇对人胃癌SGC-7901细胞中血管内皮生长因子（vascularendothelial growthfactor,VEGF）、CXC趋化因子受体4（CXCchemokinereceptor 4,CXCR4）mRNA及蛋白表达的影响。方法：采用甲基噻唑基四唑法观察氧化苦参碱注射液联合小剂量紫杉醇对人胃癌SGC-7901细胞增殖的影响;采用实时逆转录聚合酶链反应法、免疫荧光法、酶联免疫吸附法分别检测氧化苦参碱注射液联合小剂量紫杉醇对人胃癌SGC-7901细胞中VEGF、CXCR4 mRNA及蛋白表达的影响。结果：单独使用40μg/mL氧化苦参碱注射液或小剂量（20μg/mL）紫杉醇作用于SGC-7901细胞后,与对照组比较,除了20μg/mL紫杉醇对VEGF在mRNA水平无明显影响外（P〉0.05）,两者均能抑制细胞增殖,减少VEGF、CXCR4在mRNA及蛋白水平的表达（P〈0.01）;两者联用后能明显降低SGC-7901细胞VEGF、CXCR4 mRNA及蛋白的表达,与单用小剂量紫杉醇相比差异有统计学意义（P〈0.01）。结论：氧化苦参碱注射液联合小剂量紫杉醇具有明显的抑制人胃癌SGC-7901细胞VEGF和CXCR4的作用,这可能是氧化苦参碱注射液抗肿瘤血管生成的机制之一。Objective： To investigate the effects of oxymatrine injection（OI） combined with low-dose paclitaxel on expressions of mRNAs and proteins of vascular endothelial growth factor（VEGF） and CXC chemokine receptor 4（CXCR4） in human gastric carcinoma SGC-7901 cells.Methods： Methyl thiazolyl tetrazolium assay was used to examine the effects of OI combined with low-dose paclitaxel on proliferation of SGC-7901 cells.Real-time reverse transcription-polymerase chain reaction,immunofluorescence and enzyme-linked immunosorbent assay were employed to measure the expressions of VEGF and CXCR4 mRNAs and proteins in gastric carcinoma SGC-7901 cells respectively.Results： Except that 20 ？？/mL paclitaxel had no influence on expression of VEGF mRNA in SGC-7901 cells（P0.05）,40 ？？/mL OI or low-dose paclitaxel（20 ？？/mL） inhibited the proliferation of SGC-7901 cells and reduced the expressions of VEGF and CXCR4 mRNAs and proteins in SGC-7901 cells（P0.01）.The expressions of VEGF and CXCR4 mRNAs and proteins in the OI plus low-dose paclitaxel group were markedly lower than those in the low-dose paclitaxel group（P0.01）.Conclusion： OI combined with low-dose paclitaxel can inhibit VEGF and CXCR4 of gastric carcinoma SGC-7901 cells markedly,which may be one of its mechanisms of anti-angiogenic ability.

关 键 词：氧化苦参碱 紫杉醇 胃肿瘤 SGC-7901细胞 血管内皮生长因子 受体 CXCR4 

分 类 号：R735.2[医药卫生—肿瘤]