肝细胞癌细胞膜表面唾液酸含量的变化  

Variations of the amount of sialic acids on hepatocellular carcinoma cell membrane

作　　者：龚祖元[1] 廖彩仙[1] 王宇[1] 廖欣鑫[1] 秦安成[1] 黄勇平[1] 廖晖[1] 

机构地区：[1]南方医科大学南方医院肝胆外科,广东广州510515

出　　处：《南方医科大学学报》2010年第10期2323-2326,共4页Journal of Southern Medical University

基　　金：广州市科技计划攻关课题(2007Z3-E0371)

摘　　要：目的观察肝细胞癌细胞膜表面唾液酸含量的变化。方法肝细胞癌组织和肝硬化肝组织从手术病人的切除标本中获取,共28例。首先采用胶原酶消化法,将癌组织和肝硬化肝组织制备成癌细胞悬液和肝细胞悬液。随后将细胞加入含荧光标记的朝鲜槐凝集素(FITC-MAL)或黑接榾凝集素(FITC-SNA)的缓冲液中孵育1h,使其充分与细胞膜表面糖链末端的α2,3-唾液酸(α2,3-SA)或α2,6-唾液酸(α2,6-SA)结合。最后用流式细胞仪检测细胞表面的荧光强度,并据此评定细胞膜表面α2,3或α2,6-唾液酸的含量。结果 FITC-MAL孵育组和FITC-SNA孵育组的癌细胞表面荧光强度均显著大于肝细胞表面的荧光强度。结论肝硬化组织肝细胞发生癌变后,细胞膜表面α2,3和α2,6-唾液酸含量均显著增加。Objective To observe the change in the amount of sialic acids on hepatocellularcarcinoma（HCC） cell membrane.Methods Surgical specimens of HCC and livercirrhosis tissues were obtained from 28 patients to prepare carcinoma cell and hepatocyte suspensions by collagenase digestion.For assay of ？？,3 and ？？,6-sialic acids,the cells were suspended in the staining buffer containing either fluorescein isothiocyanate-Maackia amurensis lectin（FITC-MAL） or fluorescein isothiocyanate-Sambucus nigra bark lectin（FITC-SNA） and incubated for1 h,respectively.Flow cytometric analysis was carried out to measure the mean fluorescence intensity（MFI） on the cell surface.Results In both FITC-MAL-and FITC-SNA-incubated HCC cells,the MFI on the cell surface was greaterthan that of the hepatocytes.Conclusion Both of ？？,3 and ？？,6-sialic acids increases significantly on the hepatocyte membrane afterthe carcinomatous change.

关 键 词：肝细胞癌 肝硬化 唾液酸 

分 类 号：R735.7[医药卫生—肿瘤]