间充质干细胞培养与分化诱导的特点及机制  

Characteristics and mechanisms of mesenchymal stem cell culture and induced differentiation

作　　者：赵林[1] 申延清[1] 荣春[1] 周岩冰[2] 夏长所[3] 

机构地区：[1]青岛大学医学院附属医院手术室,山东省青岛市266003 [2]青岛大学医学院附属医院普外科,山东省青岛市266003 [3]青岛大学医学院附属医院骨科,山东省青岛市266003

出　　处：《中国组织工程研究与临床康复》2010年第40期7551-7554,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：青岛大学医学院附属医院博士科研基金(博2006-21);课题名称"干细胞的基础与临床应用研究"~~

摘　　要：背景:间充质干细胞是目前备受关注的一类具有多向分化潜能的成体干细胞。目的:对间充质干细胞分离、鉴别、生物学特性及应用前景做一综述。方法:应用计算机检索Medline数据库(1996-01/2010-03),以"mese nchymal stem cell"为检索词;同时检索CNKI数据库(1979/2010-02),以"间充质干细胞、培养、分化"为检索词。结果与结论:共收集400篇关于间充质干细胞分离、培养、鉴定和分化的文献,中文86篇,英文314篇。排除发表时间较早、重复及类似研究,纳入36篇符合标准的文献。发现间充质干细胞分离、培养、鉴定虽然有多种方法,但还没有统一的标准,细胞分化诱导的机制仍不清楚,应用也存在诸多问题。BACKGROUND：Mesenchymal stem cells（MSCs） are a class of adult stem cells with multi-directional differentiation potential.OBJECTIVE：To review MSCs separation,identification,characterization and application prospects.METHODS：The Medline database was searched for articles published from January 1996 to March 2010.The key words were ＂mesenchymal stem cells＂.The China National Knowledge Infrastructure database was retrieved for articles published from 1979 to February 2010.The key words were ＂mesenchymal stem cells,culture,differentiation＂.RESULTS AND CONCLUSION：A total of 400 articles concerning MSCs isolation,culture,identification and differentiation were collected,including 86 Chinese articles and 314 English articles.Published earlier,repeated articles and those with similar studies were excluded,and 36 articles in accordance with inclusion criteria were included.We found that there are many methods concerning MSCs isolation,culture and identification,but there is no uniform standard,the mechanism of inducing cell differentiation remains unclear,and there are many problems in application.

关 键 词：间充质干细胞 培养 分化 诱导 综述文献 

分 类 号：R394.2[医药卫生—医学遗传学]