添加外源脂肪酶对瓦氏黄颡鱼的生长、消化酶及血清生化指标的影响  

Effect of adding lipase in diet on growth performance, digestive enzyme and serum biochemical indexes of Pseudobagru vachelli

作　　者：谷金皇[1] 杨毅[1,2] 冷向军[1] 吴江 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]通威股份水产工程技术中心,四川成都610081

出　　处：《上海海洋大学学报》2010年第6期798-804,共7页Journal of Shanghai Ocean University

基　　金：上海市重点学科建设项目(Y1101)

摘　　要：为考察添加脂肪酶酶制剂对瓦氏黄颡鱼生长、消化酶以及血清生化指标的影响,在基础饲料中分别添加0mg/kg、100mg/kg、300mg/kg、500mg/kg脂肪酶制剂,饲喂平均体重(3.47±0.05)g的瓦氏黄颡鱼90d。试验结果表明:添加脂肪酶100mg/kg、300mg/kg、500mg/kg组分别比对照组增重率提高3.86%(P>0.05)、9.29%(P<0.05)、6.75%(P>0.05),饲料系数降低了3.17%(P>0.05)、6.35%(P<0.05)、5.29%(P<0.05)。同时添加脂肪酶各组的血清甘油三酯、低密度脂蛋白和总胆固醇含量都显著低于对照组(P<0.05),通过消化酶测定表明,脂肪酶添加各组均显著提高了胰蛋白酶和肠淀粉酶活性(P<0.05),100mg/kg脂肪酶组还显著提高了胰脂肪酶和胰淀粉酶(P<0.05),300mg/kg脂肪酶组胰脂肪酶活性最高(P<0.05)。上述研究表明,脂肪酶在瓦氏黄颡鱼饲料中的适当添加量为300mg/kg为宜。This study was conducted to evaluate the effect of lipase enzyme preparation additive to Pseudobagru vachelli diet on growth performance,digestive enzyme and serum biochemical indexes.P.vachelli with the average initial weight of（3.47 ± 0.05） g were randomly divided into four groups and fed on diets added with 0,100 mg /kg,300 mg /kg and 500 mg /kg lipase for 90 days.The test results showed that： growth rate of 100 mg /kg,300 mg /kg and 500 mg /kg lipase additive groups was increased by 3.86% （P〉0.05） ,9.29% （P〈0.05） ,6.75% （P〉0.05） ,and the coefficient of feed decreased by 3.17% （P〉0.05） ,6.35% （P〈0.05 ） ,5.29% （P〈0.05 ） .Compared with control group,the addition of lipase significantly lowered the contents of triglyceride and total cholesterol,low-density lipoprotein cholesterol in blood（P〈0.05） .The lipase activity of midintestine and trypsin activity were significantly higher than those of the control （P〈0.05 ） .Meanwhile,the lipase and amylase activities of hepatopancreas significantly improved in the group with addition of 100 mg /kg lipase（P〈0.05） .The lipase activity of hepatopancreas was highest with 300 mg /kg lipase additive.In conlusion,it showed that the addition of about 300 mg /kg might be helpful for improving the growth performance of P.vachelli.

关 键 词：瓦氏黄颡鱼 脂肪酶 生长 消化酶 血清生化指标 

分 类 号：S963.1[农业科学—水产养殖]