基于单克隆抗体的酶联免疫法与高效液相色谱法检测水产品中环丙沙星残留的比较  

Comparing the competitive indirect enzyme-linked immunosorbent assay based on monoclonal antibody and High Performance Liquid Chromatography methods to determinate the ciprofloxacin residues in fishery products

作　　者：胡鲲[1] 黄宣运[1] 姜有声[1] 方伟[2] 朱泽闻[3] 杨先乐[1] 

机构地区：[1]上海海洋大学农业部渔业动植物病原库,上海201306 [2]广东省水生动物疫病预防控制中心,广东广州510222 [3]全国水产技术推广总站,北京100026

出　　处：《上海海洋大学学报》2010年第6期805-809,共5页Journal of Shanghai Ocean University

基　　金：上海市科技兴农重点攻关项目(6660106477);公益性行业(农业)科研专项(200803013);公益性行业(农业)科研专项(nyhyzx07-042);现代农业产业技术体系建设专项资金(NYCYTX-49-17)

摘　　要：为了快速、灵敏地检测禁用药物环丙沙星在水产品中的残留,建立了基于单克隆抗体的间接竞争酶联免疫法(competitive indirect ELISA,ci-ELISA),并比较了其与高效液相色谱法(High Performance LiquidChromatography,HPLC)在灵敏度、准确性和重现性等方法学参数上的差异。实验结果显示,经过浓缩、净化等前处理过程,ci-ELISA与HPLC的检测灵敏度分别可以达到10μg/kg和1μg/kg,回收率分别为79.28%～92.92%和86.27%～97.32%,相对标准偏差分别在3.21%～8.88%和2.66%～5.52%。前者经济、高效,适合作为初筛方法大批量筛选样品;后者灵敏度、重现性更好,适于在实验室条件下作为验证方法。For sensitive and specifically detection of ciprofloxacin （CIP） residues in fishery products,this study is aimed at generating and developing a competitive indirect enzyme-linked immunosorbent assay （ciELISA） based on CIP-specific monoclonal antibodies （McAb） .The sensibility,accuracy and repeatability of ci-ELISA method was compared to those of High Performance Liquid Chromatography（HPLC） method.By concentration and purification process,the limit of detection （LOD） of ci-ELISA and HPLC method could reach to 10 μg /kg and 1 μg /kg respectively.we detected the recovery rates were 79.28%-92.92% and 86.27%-97.32% while relative standard deviation （RSD） varying from 3.21%-8.88% and 2.66%-5.52% for these methods.Between the two methos,ci-ELISA method was cheaper and more efficient while HPLC method was more sensitive and stable.

关 键 词：环丙沙星 水产品 残留 间接酶联免疫法 高效液相色谱法 

分 类 号：S948[农业科学—水产养殖]